University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093 Warsaw, Poland; University of Warsaw, MISMaP College, Banacha 2C, 02-097 Warsaw, Poland.
University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
Talanta. 2018 Dec 1;190:423-428. doi: 10.1016/j.talanta.2018.08.026. Epub 2018 Aug 7.
Rapid Prototyping technologies expand the availability of fabrication of plastic objects to non-skilled users that need sophisticated equipment for their research. In this communication, for the very first time, the universal design of photometric-fluorometric, UV-Vis compatible, 3D-printed flow-through cuvette with two optical paths (2 and 10 mm) is introduced. The cuvette insert was made with the use of the most economically viable Fused Material Deposition technology which enables truly one-step manufacturing and easy replicating of the device. A utility of the cuvette was presented in the example of the basic flow injection analysis experiments on the model photometric (bromothymol blue) and fluorometric (fluorescein) dyes and proven by investigation of solubility constant of calcium hydrophosphate dihydrate by determination of phosphate using fluorescence quenching of molybdenum blue-Rhodamine B ion pair formation and calcium reaction with calcein in basic environment.
快速原型制造技术拓展了塑料物体制造的可用性，使非专业用户也能使用复杂的设备进行研究。在本次交流中，我们首次介绍了具有通用设计的光度荧光兼容、紫外可见兼容、带有两个光程（2 毫米和 10 毫米）的 3D 打印流通池。该流通池插件是使用最经济可行的熔丝制造技术制造的，这种技术可以实现真正的一步制造和设备的轻松复制。我们通过使用模型光度（溴百里酚蓝）和荧光（荧光素）染料的基本流动注射分析实验来展示该流通池的实用性，并通过使用钼蓝罗丹明 B 离子对形成和钙在碱性环境中与钙试剂的反应来荧光猝灭法测定磷酸根来验证二水合磷酸氢钙的溶度积常数。
