Hayano T, Sogawa K, Ichihara Y, Fujii-Kuriyama Y, Takahashi K
Biochem Biophys Res Commun. 1986 Jul 16;138(1):289-96. doi: 10.1016/0006-291x(86)90278-0.
We have obtained a clone containing two pepsinogen A genes in a single insert by screening a recombinant cosmid library for human genomic DNA. Restriction endonuclease mappings of this cloned DNA showed that these two genes are very similar, but distinct in structure, and that they are closely linked to one another in the human chromosome DNA. The close arrangement of the genes with very similar structures could facilitate the homologous recombination or the unequal crossing-over which accounts for high frequency of haplotype variation in copy number of pepsinogen A genes as reported by Taggart et al.
通过筛选人基因组DNA的重组黏粒文库，我们获得了一个克隆，该克隆在单个插入片段中包含两个胃蛋白酶原A基因。对该克隆DNA的限制性内切酶图谱分析表明，这两个基因非常相似，但结构不同，并且它们在人类染色体DNA中彼此紧密相连。结构非常相似的基因紧密排列可能会促进同源重组或不等交换，这正如Taggart等人所报道的，解释了胃蛋白酶原A基因拷贝数单倍型变异的高频率。
