Dimicoli J L, Jullien P, Carrez D, Croisy A, Lhoste J M
Biochem Biophys Res Commun. 1986 Jul 31;138(2):699-706. doi: 10.1016/s0006-291x(86)80553-8.
31P NMR spectra of normal rodent and avian fibroblasts were compared to those of the same cells transformed either by the Rous sarcoma virus (RSV) or by the Kirsten sarcoma virus (Ki-MSV). Under physiological conditions, the spectra of living or perchloric acid extracted chicken embryo fibroblasts, rat cell line FR3T3 and mouse cell line C127 did not differ from those of their counterparts transformed by RSV or Ki-MSV. However, in the case of FR3T3 cells, on shifting from 37 degrees C to 20 degrees C, and particularly if PBS replaced serum growth medium, a different, though transitory, response of the transformed cells was detected. They then showed, within few minutes, a more rapid ATP depletion with accumulation of fructose 1,6-diphosphate (FDP), as compared to normal control cells.
将正常啮齿动物和禽类成纤维细胞的31P NMR光谱与经劳氏肉瘤病毒（RSV）或 Kirsten 肉瘤病毒（Ki-MSV）转化的相同细胞的光谱进行了比较。在生理条件下，活的或经高氯酸提取的鸡胚成纤维细胞、大鼠细胞系FR3T3和小鼠细胞系C127的光谱与经RSV或Ki-MSV转化的对应细胞的光谱没有差异。然而，对于FR3T3细胞，当从37℃转移到20℃时，特别是如果用PBS代替血清生长培养基，会检测到转化细胞有不同的、尽管是短暂的反应。与正常对照细胞相比，它们在几分钟内就显示出ATP更快地消耗，同时果糖1,6-二磷酸（FDP）积累。
