Variane Augusto Carlos Favaretto, Santos Fabiane Cristina Dos, Castro Fausto Fernandes de, Barbosa-Tessmann Ione Parra, Santos Geraldo Tadeu Dos, Pozza Magali Soares Dos Santos
Universidade Estadual de Maringá, Departamento de Zootecnia, Maringá, PR, Brazil.
Universidade Estadual de Maringá, Departamento de Bioquímica, Maringá, PR, Brazil.
Braz J Microbiol. 2018 Oct-Dec;49(4):919-928. doi: 10.1016/j.bjm.2018.05.005. Epub 2018 Aug 14.
The presence of mycotoxins or related fungi in animal feed is a major problem for animal and human health. Silage and concentrated feed samples were collected from 21 dairy farms in the Western part of Paraná state in Southern Brazil. Water activity and pH of all samples were measured, and each sample was analyzed to check for the presence of aflatoxigenic Aspergillus. Water activity was observed to be lower in the concentrated feed samples. The pH was lower in the silage samples, indicating fermentation processes. Two silage samples and four concentrated feed samples were contaminated with Aspergillus spp. Seven isolates of Aspergillus spp. were obtained and their potential to produce aflatoxins was evaluated. Four of the isolates, two from the silage samples and two from the concentrated feed samples, produced the aflatoxins B1, B2, G1, and G2 in culture media. These isolates were identified as Aspergillus parasiticus and Aspergillus nomius. The presence of aflatoxigenic isolates of Aspergillus spp. in silage and concentrated feed samples is a matter of concern, because of the risk of aflatoxin production and contamination of the animal feed.
动物饲料中存在霉菌毒素或相关真菌是影响动物和人类健康的一个主要问题。从巴西南部巴拉那州西部的21个奶牛场采集了青贮饲料和浓缩饲料样本。测量了所有样本的水分活度和pH值，并对每个样本进行分析以检测产黄曲霉毒素的曲霉菌的存在情况。观察到浓缩饲料样本中的水分活度较低。青贮饲料样本的pH值较低，表明存在发酵过程。两个青贮饲料样本和四个浓缩饲料样本被曲霉菌属污染。获得了七株曲霉菌属分离株，并评估了它们产生黄曲霉毒素的潜力。其中四株分离株，两株来自青贮饲料样本，两株来自浓缩饲料样本，在培养基中产生了黄曲霉毒素B1、B2、G1和G2。这些分离株被鉴定为寄生曲霉和诺米曲霉。青贮饲料和浓缩饲料样本中存在产黄曲霉毒素的曲霉菌属分离株令人担忧，因为存在产生黄曲霉毒素和污染动物饲料的风险。
