Le Parco Y, Cecchini J P, Knibiehler B, Mirre C
Biol Cell. 1986;56(3):217-26. doi: 10.1111/j.1768-322x.1986.tb00453.x.
We have used a cloned chicken collagen cDNA sequence to help identify hypothetic members of the collagen gene family from Drosophila melanogaster. Several experimental evidences have been obtained which indicate that the Drosophila genome contains numerous collagen-like sequences. We have characterized in more detail ten distinct DNA sequences that hybridized strongly to the heterologous collagen probe. By in situ hybridization we have shown that these sequences are dispersed throughout the Drosophila genome. Two of them are shown to originate from the previously described DCg 1 and DCg 2 collagen genes. In other respects, we show that in addition to DCg 1 and DCg 2, at least five putative collagen genes are expressed during the Drosophila lifetime. These genes are unique, and some of them are seen to be transcribed into different size classes of mRNAs. Additionally, the data presented so far demonstrate that the expression of these genes is regulated temporally and/or quantitatively during the Drosophila life cycle.
我们利用克隆的鸡胶原蛋白cDNA序列，来帮助鉴定黑腹果蝇胶原蛋白基因家族的假定成员。已获得的若干实验证据表明，果蝇基因组包含众多类胶原蛋白序列。我们更详细地鉴定了十个与异源胶原蛋白探针强烈杂交的不同DNA序列。通过原位杂交，我们表明这些序列分散在果蝇基因组中。其中两个序列显示源自先前描述的DCg 1和DCg 2胶原蛋白基因。在其他方面，我们表明，除了DCg 1和DCg 2之外，果蝇一生中至少还有五个假定的胶原蛋白基因表达。这些基因是独特的，其中一些被发现转录成不同大小类别的mRNA。此外，目前呈现的数据表明，这些基因的表达在果蝇生命周期中受到时间和/或定量调控。
