Irving W L, Jenkins R E, Walker P R, Clayden S A, Lydyard P M
Cell Immunol. 1985 Dec;96(2):245-54. doi: 10.1016/0008-8749(85)90357-0.
Direct addition of the T-cell mitogen, concanavalin A (Con A), to cultures of Epstein-Barr virus (EBV)-stimulated peripheral blood mononuclear cells (PBMC) resulted in a dose-dependent inhibition of immunoglobulin M (IgM) secreted in the supernatant, as measured by an enzyme-linked immunosorbent assay. Furthermore, Con A inhibited IgM secretion of isolated T-depleted cells stimulated with EBV, and both the proliferation and IgM secretion of EBV-driven lymphoblastoid cell lines. T-Enriched cells, precultured for 48 hr with Con A, were also able to suppress the IgM response of fresh autologous PBMC stimulated with EBV. This suppression was radiation sensitive (2000 rad), a procedure which resulted in enhancement of the IgM secretion of the responder cells in two out of three experiments. Studies on the long-term effects of Con A showed that the early suppression of IgM secretion was transient and that the mitogen prevented the development of the cytotoxic T-cell response normally seen with lymphocytes from EBV-seropositive donors after 5 weeks of culture. Thus, Con A appears to modulate human lymphocyte responses to EBV by multiple mechanisms.
将T细胞促有丝分裂原刀豆蛋白A（Con A）直接添加到爱泼斯坦-巴尔病毒（EBV）刺激的外周血单个核细胞（PBMC）培养物中，通过酶联免疫吸附测定法测量，结果显示上清液中分泌的免疫球蛋白M（IgM）受到剂量依赖性抑制。此外，Con A抑制了EBV刺激的分离的T细胞耗竭细胞的IgM分泌，以及EBV驱动的淋巴母细胞系的增殖和IgM分泌。用Con A预培养48小时的富含T细胞的细胞，也能够抑制EBV刺激的新鲜自体PBMC的IgM反应。这种抑制对辐射敏感（2000拉德），在三个实验中有两个实验中，该过程导致反应细胞的IgM分泌增强。对Con A长期影响的研究表明，IgM分泌的早期抑制是短暂的，并且该促有丝分裂原阻止了通常在培养5周后EBV血清阳性供体的淋巴细胞中出现的细胞毒性T细胞反应的发展。因此，Con A似乎通过多种机制调节人淋巴细胞对EBV的反应。
