Vitiello A, Nespoli L, Lanzavecchia A, Maccario R, Ugazio A G
Thymus. 1980 Jan;1(4-5):215-23.
Tonsillar lymphocytes cultured for 7 days in serum-supplemented RPMI-1640 medium were stimulated with Con A or PWM. IgM production was assessed by a hemagglutination inhibition assay. IgM was produced after Con A as well as PWM stimulation. Our data show that T-cell irradiation and/or addition of small numbers of T cells to the responding B-cell population facilitate(s) IgM synthesis. In particular in Con A-stimulated cultures the T-cell number is critical in regulating the appearance of suppressor effects. The results are discussed in terms of the various subpopulations possibly involved in the response to these mitogens.
在补充血清的RPMI-1640培养基中培养7天的扁桃体淋巴细胞，用刀豆蛋白A（Con A）或美洲商陆有丝分裂原（PWM）刺激。通过血凝抑制试验评估IgM的产生。Con A和PWM刺激后均产生了IgM。我们的数据表明，对反应性B细胞群体进行T细胞照射和/或添加少量T细胞可促进IgM合成。特别是在Con A刺激的培养物中，T细胞数量对于调节抑制作用的出现至关重要。根据可能参与对这些有丝分裂原反应的各种亚群对结果进行了讨论。
