Miuller S, Stenina M A
Biull Eksp Biol Med. 1977 Jun;83(6):714-6.
Preliminary treatment of erythrocyte suspension with trypan blue solution did not affect their capacity to participate in the reaction of spontaneous rosette formation, but permitted to differentiate them in a mixture with other erythrocytes after staining with methyl-green-pyronine on a fixed slide. By using a mixture of such marked erythrocytes sensitized with antibodies and a complement, the presence in the peripheral blood of healthy people of a small lymphocyte subpopulation connecting these two types of indicator particles was demonstrated.
用台盼蓝溶液对红细胞悬液进行预处理，并不影响其参与自发玫瑰花结形成反应的能力，但在用甲基绿-派洛宁对固定载玻片上的细胞进行染色后，可使其在与其他红细胞的混合物中得以区分。通过使用经抗体和补体致敏的此类标记红细胞的混合物，证实了健康人外周血中存在连接这两种指示颗粒的小淋巴细胞亚群。
