Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, South Korea.
Agricultural Microbiology Division, National Academy of Agricultural Science, RDA, Wanju, South Korea.
Pest Manag Sci. 2019 Mar;75(3):852-858. doi: 10.1002/ps.5191. Epub 2018 Oct 11.
An entomopathogenic fungus, Isaria javanica pf185, causes mortality in nymphs of the green peach aphid and inhibits the growth of fungal plant pathogens. However, the metabolites of pf185 involved in these antifungal and aphicidal activities are unknown. This study was performed to identify the metabolites with these activities.
An antifungal metabolite was purified by repetitive column chromatography and preparative high-performance liquid chromatography. Based on data from mass spectrometry and nuclear magnetic resonance, the active metabolite was identified as dibutyl succinate. The minimum concentration of dibutyl succinate to inhibit germination of conidia of the cause of anthracnose, Colletotrichum acutatum, was 4 mg mL . Dibutyl succinate at 2 µg mL inhibited mycelial growth. It also had strong aphicidal activity against the nymphs of the green peach aphid, Myzus persicae, median lethal concentration (LC50) of 306 mg L at 24 h exposure, and median lethal time (LT50) of 14.5 h at 388 mg L exposure.
This is the first report indicating that a single metabolite, dibutyl succinate, from the beneficial fungus I. javanica, has potential for use in integrated pest management to inhibit both insect and fungal plant pathogens. © 2018 Society of Chemical Industry.
一种昆虫病原真菌，淡紫拟青霉 pf185，可导致桃蚜若虫死亡，并抑制真菌植物病原体的生长。然而，pf185 产生的参与这些抗真菌和杀蚜活性的代谢物尚不清楚。本研究旨在鉴定具有这些活性的代谢物。
通过重复柱层析和制备高效液相色谱法纯化了一种具有抗真菌活性的代谢物。根据质谱和核磁共振数据，该活性代谢物被鉴定为丁二酸二丁酯。丁二酸二丁酯抑制炭疽病病原菌胶孢炭疽菌分生孢子萌发的最低浓度为 4 mg mL 。丁二酸二丁酯在 2 µg mL 时抑制菌丝生长。它对桃蚜若虫也具有很强的杀蚜活性，24 h 暴露时的半数致死浓度（LC50）为 306 mg L ，在 388 mg L 暴露时的半数致死时间（LT50）为 14.5 h。
这是首次报道表明，有益真菌淡紫拟青霉 pf185 中的单一代谢物丁二酸二丁酯具有抑制昆虫和真菌植物病原体的潜力，可用于综合虫害管理。© 2018 化学工业协会。
