Kang Beom Ryong, Han Ji Hee, Kim Jeong Jun, Kim Young Cheol
Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA, Wanju, Republic of Korea.
Mycobiology. 2018 Dec 21;46(4):440-447. doi: 10.1080/12298093.2018.1538073. eCollection 2018.
Dual biocontrol of both insects and plant pathogens has been reported for certain fungal entomopathogens, including and spp. In this study, we demonstrate, for the first time, the dual biocontrol potential of two fungal isolates identified by morphological and phylogenetic analyses as . Both these isolates caused mortality in the greater wax moth, and hence can be considered entomopathogens. Spores of the isolates were also pathogenic to nymphs of the green peach aphid (), with an LC value of 10 spores/mL 4 days after inoculation and an LT of 4.2 days with a dose of 10 spores/mL. antifungal assays also demonstrated a strong inhibitory effect on the growth of two fungi that are pathogenic to peppers, and . These results indicate that isolates could be used as novel biocontrol agents for the simultaneous control of aphids and fungal diseases, such as anthracnose and Phytophthora blight, in an integrated pest management framework for red pepper.
据报道，某些真菌性昆虫病原体，包括白僵菌属和绿僵菌属的一些种，对昆虫和植物病原体具有双重生物防治作用。在本研究中，我们首次证明了通过形态学和系统发育分析鉴定为金龟子绿僵菌的两种真菌分离株具有双重生物防治潜力。这两种分离株均导致大蜡螟死亡，因此可被视为昆虫病原体。分离株的孢子对桃蚜若虫也具有致病性，接种后4天的LC50值为10⁶孢子/毫升，剂量为10⁶孢子/毫升时的LT₅₀为4.2天。抗真菌试验还表明，它们对两种辣椒致病真菌，即辣椒炭疽病菌和辣椒疫霉，具有强烈的抑制作用。这些结果表明，金龟子绿僵菌分离株可作为新型生物防治剂，在辣椒综合害虫管理框架中同时防治蚜虫和真菌病害，如炭疽病和疫病。
