Lee Yong-Seong, Kim Young Cheol
Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Mycobiology. 2019 Jan 19;47(1):126-133. doi: 10.1080/12298093.2018.1562692. eCollection 2019 Mar.
pf185 is an important entomopathogenic fungus with potential for use as an agricultural biocontrol agent. However, the effect of pf185 on plant growth is unknown. Enhanced tobacco growth was observed when tobacco roots were exposed to spores, cultures, and fungal cell-free culture supernatants of this fungus. Tobacco seedlings were also exposed to the volatiles of pf185 using I-plates in which the plant and fungus were growing in separate compartments connected only by air space. The length and weight of seedlings, content of leaf chlorophyll, and number of root branches were significantly increased by the fungal volatiles. Heptane, 3-hexanone, 2,4-dimethylhexane, and 2-nonanone were detected, by solid-phase micro-extraction and gas chromatography-mass spectrophotometry, as the key volatile compounds produced by pf185. These findings illustrate that pf185 can be used to promote plant growth, and also as a biocontrol agent of insect and plant diseases. Further studies are necessary to elucidate the mechanisms by which pf185 promotes plant growth.
pf185是一种重要的昆虫病原真菌，具有作为农业生物防治剂的潜力。然而，pf185对植物生长的影响尚不清楚。当烟草根暴露于该真菌的孢子、培养物和无真菌细胞的培养上清液时，观察到烟草生长增强。还使用I型平板让烟草幼苗暴露于pf185的挥发物中，在该平板中植物和真菌在仅通过空气空间连接的单独隔室中生长。真菌挥发物显著增加了幼苗的长度和重量、叶片叶绿素含量以及根分支数量。通过固相微萃取和气相色谱-质谱联用检测到庚烷、3-己酮、2,4-二甲基己烷和2-壬酮是pf185产生的关键挥发性化合物。这些发现表明pf185可用于促进植物生长，也可作为昆虫和植物病害的生物防治剂。有必要进一步研究以阐明pf185促进植物生长的机制。
