Kelly J M, Porter A C, Chernajovsky Y, Gilbert C S, Stark G R, Kerr I M
EMBO J. 1986 Jul;5(7):1601-6. doi: 10.1002/j.1460-2075.1986.tb04402.x.
An intact interferon-inducible gene has been isolated from a cosmid library of human genomic DNA. The gene (designated 6-16) encodes a mRNA of approximately 1 kb which is induced well by alpha- and beta- but poorly by gamma-interferons. Genomic and cDNA sequences indicate that the gene contains five exons, and that the mRNA encodes a hydrophobic polypeptide of 130 amino acids with a putative NH2-terminal signal sequence. The 5' end has been identified by primer extension. The corresponding genomic DNA contains a TATA box 20 nucleotides upstream of the putative transcription initiation site. After transfection of the human genomic cosmid into mouse Ltk- cells, human 6-16 mRNA is expressed in response to mouse alpha- and beta- but not gamma-interferons with the same kinetics and dose-response as in the human cells. No such expression is observed in response to human interferons. It can be concluded that the human cosmid DNA contains all of the sequences necessary for alpha- and beta-interferon-induced gene expression and that the mechanisms governing such expression are conserved between murine and human cells.
一个完整的干扰素诱导基因已从人类基因组DNA的黏粒文库中分离出来。该基因（命名为6-16）编码一个约1 kb的mRNA，它被α-和β-干扰素强烈诱导，但被γ-干扰素诱导的程度较弱。基因组和cDNA序列表明该基因含有五个外显子，且该mRNA编码一个含假定氨基末端信号序列的130个氨基酸的疏水多肽。5'端已通过引物延伸法确定。相应的基因组DNA在假定转录起始位点上游20个核苷酸处含有一个TATA框。将人类基因组黏粒转染到小鼠Ltk-细胞后，人类6-16 mRNA会响应小鼠的α-和β-干扰素而表达，但不响应γ-干扰素，其动力学和剂量反应与在人类细胞中相同。对人类干扰素无此表达。可以得出结论，人类黏粒DNA包含α-和β-干扰素诱导基因表达所需的所有序列，且控制这种表达的机制在小鼠和人类细胞之间是保守的。
