China MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
National Reference Laboratory of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, China.
Front Cell Infect Microbiol. 2018 Aug 20;8:285. doi: 10.3389/fcimb.2018.00285. eCollection 2018.
is considered as the leading cause of gastroenteritis all over the world. This bacterium has the CRISPR- system, which is used as a gene editing technique in different organisms. However, its role in bacterial virulence has just been discovered; that discovery, however, is just the tip of the iceberg. The purpose of this study is to find out the relationship between and virulence both phenotypically and genotypically in . NCTC11168. Understanding both aspects of this relationship allows for a much deeper understanding of the mechanism of bacterial pathogenesis. The present study determined virulence in wild and mutant strains by observing biofilm formation, motility, adhesion and invasion, intracellular survivability, and cytotoxin production, followed by the transcriptomic analysis of both strains. The comparative gene expression profile of wild and mutant strains was determined on the basis of De-Seq transcriptomic analysis, which showed that the gene is involved in enhancing virulence. Differential gene expression analysis revealed that multiple pathways were involved in virulence, regulated by the CRISPR- system. Our findings help in understanding the potential role of in regulating the other virulence associated genes in NCTC11168. The findings of this study provide critical information about 's potential involvement in enhancing the virulence of , which is a major public health threat.
被认为是全世界范围内导致胃肠炎的主要原因。这种细菌拥有 CRISPR 系统，该系统被用作不同生物体中的基因编辑技术。然而，其在细菌毒力中的作用刚刚被发现；然而，这仅仅是冰山一角。本研究旨在从表型和基因型两方面探讨 在 中与毒力的关系。NCTC11168。深入了解这种关系的两个方面，可以让我们更深入地了解细菌发病机制。本研究通过观察生物膜形成、运动性、黏附与侵袭、细胞内生存能力和细胞毒素产生，来确定野生型和突变菌株的毒力，然后对两种菌株进行转录组分析。基于 De-Seq 转录组分析，确定了野生型和突变型菌株的比较基因表达谱，结果表明 基因参与增强毒力。差异基因表达分析显示，多个通路参与了毒力的调控，由 CRISPR-系统调控。我们的研究结果有助于理解 在调节 NCTC11168 中其他与毒力相关基因方面的潜在作用。这项研究的结果提供了关于 在增强 的毒力方面的潜在作用的重要信息，这是一个主要的公共卫生威胁。
