China MOA Laboratory for Risk Assessment of Quality & Safety of Livestock & Poultry Products, Huazhong Agricultural University, Wuhan 430070, PR China.
National Reference Laboratory of Veterinary Drug Residues & MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, PR China.
Future Microbiol. 2018 Dec;13:1757-1774. doi: 10.2217/fmb-2018-0234. Epub 2018 Dec 11.
The purpose of current study is to find out relationship between cas9 gene and antimicrobial resistance in Campylobacter jejuni NCTC11168.
MATERIALS & METHODS: The involvement of the cas9 gene in antimicrobial resistance of C. jejuni was determined by assessment of minimum inhibitory concentration, clustered regularly interspaced short palindromic repeats (CRISPR)-cas gene expression in standard strains, in vitro resistance development and transcriptome analysis of a cas9 deletion mutant and wild strains.
Increased expression of CRISPR-related genes was observed in standard strains. We also observed that Δcas9 mutant strain is more sensitive to antibiotics than its wild strain. Transcriptome analysis revealed that cas9 gene regulate several genes to promote antimicrobial resistance in C. jejuni.
CRISPR-cas system plays role in the enhancement of antimicrobial resistance in C. jejuni.
本研究旨在探讨 Cas9 基因与空肠弯曲菌 NCTC11168 中抗菌药物耐药性的关系。
通过评估最小抑菌浓度、标准菌株中 CRISPR-cas 基因表达、体外耐药发展和 cas9 缺失突变株和野生株的转录组分析，确定 Cas9 基因在空肠弯曲菌抗菌药物耐药性中的作用。
标准菌株中观察到 CRISPR 相关基因表达增加。我们还观察到Δcas9 突变株比其野生株对抗生素更敏感。转录组分析表明，cas9 基因调节多个基因，以促进空肠弯曲菌的抗菌药物耐药性。
CRISPR-cas 系统在增强空肠弯曲菌的抗菌药物耐药性中发挥作用。
