Matsuura Yuu, Matsubayashi Makoto, Nukata Satoko, Shibahara Tomoyuki, Ayukawa Osamu, Kondo Yasuko, Matsuo Tomohide, Uni Shigehiko, Furuya Masaru, Tani Hiroyuki, Tsuji Naotoshi, Sasai Kazumi
Acta Parasitol. 2017 Mar 1;62(1):214-220. doi: 10.1515/ap-2017-0026.
In the production and management of beef and dairy cattle, controlling diarrhea is one of the important concerns. Pathogenic agents of the disease, protozoan parasites including Cryptosporidium spp., are difficult to control, making prevention, diagnoses, and treatment of diarrhea. In the present study, we investigated a farm with a history of calf deaths over a period of 10 years in order to determine the cause of disease and to clarify the detailed distribution of the pathogens. In four examined calves that were reared in calf pens, all were positive with Cryptosporidium and/or Giardia, while the other breeding stock and adult cattle were negative. Molecular analyses revealed that the isolates from calves were C. parvum subtype IIaA15G2R1 as a zoonotic and G. intestinalis assemblage E. Other pathogenic bacteria and diarrhea-causing viruses were not detected. After treating the calf pens with boiling water and milk of lime (Ca[OH]2), oocysts of C. parvum and cysts of G. intestinalis were not found and no additional calves died. This is the first report to describe the mixed infection of both parasites in Japan.
在肉牛和奶牛的生产与管理中，控制腹泻是重要关注点之一。该疾病的病原体，包括隐孢子虫属在内的原生动物寄生虫，难以控制，这给腹泻的预防、诊断和治疗带来困难。在本研究中，我们调查了一个有10年犊牛死亡史的农场，以确定病因并阐明病原体的详细分布情况。在四头饲养在犊牛栏中的受试犊牛中，所有犊牛隐孢子虫和/或贾第虫检测均呈阳性，而其他种畜和成年牛检测为阴性。分子分析显示，犊牛分离株为作为人畜共患病原体的微小隐孢子虫IIaA15G2R1亚型和肠贾第虫E群。未检测到其他病原菌和致腹泻病毒。用沸水和石灰乳（Ca[OH]₂）处理犊牛栏后未发现微小隐孢子虫卵囊和肠贾第虫包囊，且无额外犊牛死亡。这是日本首次描述这两种寄生虫混合感染的报告。
