Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China; Consejo Superior de Investigaciones Científicas (CSIC), Global Ecology Unit, Centre for Ecological Research and Forestry Applications (CREAF)-CSIC-Universitat Autonoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Catalonia, Spain; CREAF, Cerdanyola del Vallès, 08193 Barcelona, Catalonia, Spain.
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China.
Sci Total Environ. 2019 Jan 10;647:1113-1120. doi: 10.1016/j.scitotenv.2018.07.454. Epub 2018 Jul 31.
The direct application of inorganic-phosphate-solubilizing bacteria (iPSBs) for improving the efficiency of phosphorus (P) use leads to a low rate of bacterial survival. Biochar is a good inoculum carrier for microbial survival, and diverse feedstocks can have different effects. We generated an iPSB community using seven selected iPSB strains with various phylogenic taxonomies and P-solubilizing abilities. Biochar was then inoculated with the iPSB community and applied to soil in pots seeded with rape (Brassica napus). Growth of the rape for four weeks and the effects of biochars produced from six raw feedstocks, rice straw, rice husks, soybean straw, peanut shells, corn cobs and wood, were compared. The synthetic iPSB community had a larger capacity to solubilize inorganic P and exude organic anions than any of the individual strains. The structure of the iPSB community was analyzed by high-throughput sequencing four weeks after inoculation. All seven iPSB strains were detected, dominated by Arthrobacter defluvii 06-OD12. The abundance of the iPSB community was significantly correlated with rape biomass, P content and P uptake (P < 0.05). The biochar amendments conferred 6.86-24.24% survival of the iPSB community, with the straw biochars conferring the highest survival. The available-P content of the biochar rather than soil pH was the dominant factor for iPSB community structure, suggesting that the biochar material was critical for the survival and functioning of the iPSB community. Our study demonstrates the feasibility of biochar-assisted iPSB improvement of crop growth and P uptake.
解无机磷细菌（iPSB）直接应用于提高磷（P）利用效率会导致细菌存活率低。生物炭是微生物存活的良好接种载体，不同的原料会产生不同的效果。我们使用具有不同系统发育分类和溶磷能力的 7 种选定的 iPSB 菌株生成了一个 iPSB 群落。然后将 iPSB 群落接种到生物炭中，并将其应用于播种油菜（Brassica napus）的盆栽土壤中。比较了油菜生长四周和六种原料（稻草、稻壳、大豆秸秆、花生壳、玉米芯和木材）生产的生物炭的效果。合成的 iPSB 群落比任何单一菌株都具有更大的无机磷溶解能力和有机阴离子分泌能力。接种后四周通过高通量测序分析 iPSB 群落的结构。所有 7 种 iPSB 菌株均被检测到，其中以 Arthrobacter defluvii 06-OD12 为主导。iPSB 群落的丰度与油菜生物量、P 含量和 P 吸收量显著相关（P < 0.05）。生物炭的添加使 iPSB 群落的存活率提高了 6.86-24.24%，其中秸秆生物炭的存活率最高。生物炭的有效-P 含量而不是土壤 pH 是 iPSB 群落结构的主要因素，这表明生物炭材料对 iPSB 群落的存活和功能至关重要。我们的研究证明了生物炭辅助 iPSB 提高作物生长和 P 吸收的可行性。
