Division of Allergology , Paul-Ehrlich-Institut , Paul-Ehrlich-Straße 51-59 , 63225 Langen , Germany.
J Agric Food Chem. 2018 Sep 26;66(38):9889-9894. doi: 10.1021/acs.jafc.8b03657. Epub 2018 Sep 13.
In food allergy, a common immunological disease with a potentially severe outcome, a causative cure is not available. Correct ingredient labeling and risk assessment of unlabeled allergen cross-contact is a prerequisite for effective allergen avoidance. Specific and sensitive analytical methods, which allow for unequivocal identification and accurate quantification of allergenic components, are important tools in allergen risk management. Both protein- and DNA-based methods are in place and reveal pros and cons depending upon the application and individual analytical question. This perspective highlights relevant molecular aspects and discusses, especially, opportunities for the application of DNA-based methods for the detection of allergenic foods.
在食物过敏这一常见且可能导致严重后果的免疫性疾病中，尚无根治方法。正确的成分标签和对未贴标签过敏原交叉接触的风险评估，是有效避免过敏的前提。明确鉴定和准确定量过敏原成分的特异性和灵敏性分析方法，是过敏原风险管理的重要工具。基于蛋白质和 DNA 的方法均已存在，具体取决于应用和分析问题的不同，这些方法各有优缺点。本观点强调了相关的分子方面，并特别讨论了基于 DNA 的方法在检测致敏食品中的应用机会。
