Zhang Fang, Gao Chenshan, Bai Linlin, Chen Yiquan, Liang Shuying, Lv Xucong, Sun Jinyuan, Wang Shaoyun
College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
Beijing Laboratory of Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China.
Food Chem X. 2021 Dec 31;13:100201. doi: 10.1016/j.fochx.2021.100201. eCollection 2022 Mar 30.
Food allergy has been a serious public health problem around the world. Its prevention relies heavily on the effective avoidance of any contaminated food, making clear and accurate detection very important. LAMP is one of the most potent methods for allergen rapid detection. However, its current colorimetric readouts usually have low color contrast and narrow color variation range. Thus, here we proposed a strategy based on color evolution to enlarge the variation range as well as the contrast to improve its suitability for naked-eye observation. By simply blending two commonly used color change processes during amplification, a wider color variation window, and a near contrast color change, purple-to-green with a hues difference of 10 were obtained. Three important allergens (walnuts, hazelnuts, and peanuts) were tested with a comparable sensitivity towards fluorescent real-time LAMP. Its feasibility for practical use has also been studied. This simple but effective strategy provides a new idea for the colorimetric detection of LAMP amplicons and can be applied to various fields.
食物过敏一直是全球范围内严重的公共卫生问题。其预防很大程度上依赖于有效避免任何受污染的食物，因此清晰准确的检测非常重要。环介导等温扩增技术（LAMP）是过敏原快速检测最有效的方法之一。然而，其目前的比色读数通常颜色对比度低且颜色变化范围窄。因此，在此我们提出了一种基于颜色演变的策略，以扩大变化范围以及对比度，从而提高其裸眼观察的适用性。通过在扩增过程中简单地融合两个常用的颜色变化过程，获得了更宽的颜色变化窗口以及近乎对比的颜色变化，即紫色到绿色，色相差异为10。对三种重要的过敏原（核桃、榛子和花生）进行了测试，其灵敏度与荧光实时LAMP相当。还研究了其实际应用的可行性。这种简单但有效的策略为LAMP扩增子的比色检测提供了新思路，可应用于各个领域。
