过量表达水稻 BAHD 酰基转移酶基因可提高柳枝稷的糖化效率。

Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification.

机构信息

Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.

Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.

出版信息

BMC Biotechnol. 2018 Sep 4;18(1):54. doi: 10.1186/s12896-018-0464-8.

BACKGROUND

Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice.

RESULTS

Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues.

CONCLUSION

Our study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass.

背景

柳枝稷（Panicum virgatum L.）是一种很有前途的生物能源原料，因为它可以在贫瘠的土地上生长，并产生丰富的生物质。柳枝稷细胞壁的木质纤维素成分对酶解转化为简单糖的抗性阻碍了高效生物燃料的生产。我们之前的研究表明，过量表达 BAHD 酰基转移酶基因 OsAT10 可提高水稻的糖化效率。

结果

在这里，我们发现，在柳枝稷中过表达水稻 OsAT10 基因，可降低绿叶组织中细胞壁结合型阿魏酸（FA）的水平，在衰老组织中则降低程度较小，而在温室条件下，T 植株的绿叶组织中细胞壁结合型对香豆酸（p-CA）的水平显著增加，但在衰老组织中则降低。工程化柳枝稷系在绿色组织中的糖化效率提高了约 40%，在衰老组织中的糖化效率提高了 30%。