Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an -Acyl Homoserine Lactone (AHL)-Based Biosensor.

New approaches to deal with drug-resistant pathogenic bacteria are urgent. We studied the antibacterial effect of chitosans against an quorum sensing biosensor reporter strain and selected a non-toxic chitosan to evaluate its quorum sensing (QS) inhibition activity and its effect on bacterial aggregation. To this end, chitosans of varying degree of acetylation (DA) (12 to 69%) and molecular weight () (29 to 288 kDa) were studied. Only chitosans of low DA (~12%) inhibited bacterial growth, regardless of their . A chitosan with medium degree of polymerization (named MDP) DA30, with experimental DA 42% and 115 kDa was selected for further QS inhibition and scanning electron microscopy (SEM) imaging studies. MDP DA30 chitosan exhibited QS inhibition activity in an inverse dose-dependent manner (≤12.5 µg/mL). SEM images revealed that this chitosan, when added at low concentration (≤30.6 µg/mL), induced substantial bacterial aggregation, whereas at high concentration (234.3 µg/mL), it did not. Aggregation explains the QS inhibition activity as the consequence of retardation of the diffusion of -acylated homoserine lactones (AHLs).