Domke-Opitz I, Straub P, Kirchner H
J Virol. 1986 Oct;60(1):37-42. doi: 10.1128/JVI.60.1.37-42.1986.
Macrophages derived from human peripheral blood and cultured for 1 week were permissive for the replication of herpes simplex virus (HSV) types 1 and 2. Low titers of interferon (IFN) were produced after virus infection. The yield of infectious virions was reduced by pretreatment of cells with natural and recombinant IFN-alpha and natural IFN-beta. Recombinant and natural IFN-gamma exhibited very low antiviral activity. Treatment of cells with IFN-gamma mixed with IFN-alpha or with IFN-beta did not result in a synergistic inhibition of virus yield. We studied the synthesis of HSV type 1- and HSV type 2-coded proteins in macrophages treated with IFN-beta. Induction of the HSV beta-protein DNA polymerase was strongly inhibited in IFN-treated cells in a dose-dependent manner. As shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, other beta- and gamma-proteins of HSV were inhibited as well. Immunofluorescence studies revealed a strong inhibition of the expression of immediate early alpha-protein ICP4. The results indicate that IFN acts early during the viral replication cycle to inhibit the synthesis of HSV alpha- and beta-proteins.
源自人外周血并培养1周的巨噬细胞可允许单纯疱疹病毒1型和2型复制。病毒感染后产生低滴度的干扰素（IFN）。用天然和重组IFN-α以及天然IFN-β预处理细胞后，感染性病毒粒子的产量降低。重组和天然IFN-γ表现出非常低的抗病毒活性。用IFN-γ与IFN-α混合或与IFN-β混合处理细胞不会导致对病毒产量的协同抑制。我们研究了用IFN-β处理的巨噬细胞中单纯疱疹病毒1型和单纯疱疹病毒2型编码蛋白的合成。在IFN处理的细胞中，HSVβ蛋白DNA聚合酶的诱导以剂量依赖的方式受到强烈抑制。如十二烷基硫酸钠-聚丙烯酰胺凝胶电泳所示，HSV的其他β和γ蛋白也受到抑制。免疫荧光研究显示立即早期α蛋白ICP4的表达受到强烈抑制。结果表明，IFN在病毒复制周期早期起作用，抑制HSVα和β蛋白的合成。
