Sugimoto M, Nishi R, Ando M, Nakashima H, Araki S
Jpn J Med. 1986 May;25(2):135-43. doi: 10.2169/internalmedicine1962.25.135.
To identify the activation of alveolar macrophages as a direct measure of disease activity in pulmonary sarcoidosis and to evaluate the possible relationship with serum angiotensin-converting enzyme (ACE) levels, we studied the morphology and function of alveolar macrophages obtained by bronchoalveolar lavage from patients with active disease (n = 12) and inactive disease (n = 5), and from normal controls (n = 11). At the time of lavage, 6 of 12 patients with active disease showed an elevation of serum ACE levels. When alveolar macrophages were cultured, more cells from active disease adhered to glass, spread out, and were positive for NBT reduction (p less than 0.001). Lysosomal enzyme (beta-galactosidase) activity was higher only in patients with active disease (p less than 0.001). In active disease, there were no significant differences in morphology or function of alveolar macrophages between higher and normal serum ACE levels. Thus, alveolar macrophages were activated in active pulmonary sarcoidosis and no correlation was found with serum ACE levels. These results suggest that serum ACE levels do not accurately reflect the activity of disease in the lung.
为了确定肺泡巨噬细胞的激活作为肺结节病疾病活动的直接指标，并评估其与血清血管紧张素转换酶（ACE）水平的可能关系，我们研究了通过支气管肺泡灌洗从活动期疾病患者（n = 12）、非活动期疾病患者（n = 5）和正常对照者（n = 11）获取的肺泡巨噬细胞的形态和功能。在灌洗时，12例活动期疾病患者中有6例血清ACE水平升高。当培养肺泡巨噬细胞时，来自活动期疾病患者的更多细胞附着于玻璃、铺展并对NBT还原呈阳性（p < 0.001）。溶酶体酶（β - 半乳糖苷酶）活性仅在活动期疾病患者中较高（p < 0.001）。在活动期疾病中，血清ACE水平较高者与正常者之间肺泡巨噬细胞的形态或功能无显著差异。因此，在活动性肺结节病中肺泡巨噬细胞被激活，且未发现与血清ACE水平相关。这些结果表明血清ACE水平不能准确反映肺部疾病的活动情况。
