Biochemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
Pharmaceutical Chemistry Dept., National Organization for Drug Control and Research [NODCAR], 6 Abu Hazem Street, Pyramids Ave, P.O. Box 29, Giza, Egypt.
Mater Sci Eng C Mater Biol Appl. 2018 Nov 1;92:489-495. doi: 10.1016/j.msec.2018.06.063. Epub 2018 Jun 30.
A novel sensitive electrochemical sensor for microRNAlet-7a detection in normal serum samples, hepatocellular carcinoma patients and human liver cancer cells, has been excellently synthesized. The sensor constructed of carbon paste (CP) amended with silver nanoparticles (AgNPs) and extracted propolis (bee glue). The AgNPs/P modified carbon paste electrode (APCPE) displayed a high electrocatalytic activity in a Britton Robinson (BR) buffer (pH = 7.4). The techniques utilized to prepare this work are square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Surface characteristics were achieved using scanning (SEM), Fourier-transform infrared spectroscopy (FTIR), Spectrophotometer, transmission (TEM) electron microscope, energy dispersive X-ray analysis (EDX) and elemental mapping (EM) techniques. Under optimal conditions, the suggested sensor exhibits good rapid and sensible response reaching a very low detection limit of 10 femtomolar.
一种新型的灵敏电化学传感器，用于检测正常血清样本、肝癌患者和人肝癌细胞中的 microRNAlet-7a，已被出色地合成。该传感器由碳糊（CP）与纳米银颗粒（AgNPs）和提取的蜂胶（蜜蜂胶）组成。AgNPs/P 修饰碳糊电极（APCPE）在 Britton Robinson（BR）缓冲液（pH=7.4）中显示出高电催化活性。制备该工作所采用的技术是方波伏安法（SWV）和电化学阻抗谱（EIS）。表面特性采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、分光光度计、透射（TEM）电子显微镜、能量色散 X 射线分析（EDX）和元素映射（EM）技术来实现。在最佳条件下，所提出的传感器表现出良好的快速和灵敏响应，达到非常低的检测限 10 飞摩尔。
