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人类β-珠蛋白基因簇中的重组热点：酿酒酵母中人类DNA片段的减数分裂重组

Recombination hot spot in the human beta-globin gene cluster: meiotic recombination of human DNA fragments in Saccharomyces cerevisiae.

作者信息

Treco D, Thomas B, Arnheim N

出版信息

Mol Cell Biol. 1985 Aug;5(8):2029-38. doi: 10.1128/mcb.5.8.2029-2038.1985.

DOI:10.1128/mcb.5.8.2029-2038.1985
PMID:3018546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC366921/
Abstract

We describe a novel system for the analysis of sequence-specific meiotic recombination in Saccharomyces cerevisiae. A comparison of three adjacent restriction fragments from the human beta-globin locus revealed that one of them, previously hypothesized to contain a relative hot spot for genetic recombination, engages in reciprocal exchange during yeast meiosis significantly more frequently than either of the other two fragments. Removal of the longest of four potential Z-DNA-forming regions from this fragment does not affect the high frequency of genetic recombination.

摘要

我们描述了一种用于分析酿酒酵母中序列特异性减数分裂重组的新型系统。对来自人类β-珠蛋白基因座的三个相邻限制性片段进行比较后发现，其中一个片段（之前被假设含有遗传重组的相对热点）在酵母减数分裂过程中发生相互交换的频率明显高于其他两个片段中的任何一个。从该片段中去除四个潜在的Z-DNA形成区域中最长的一个，并不影响遗传重组的高频发生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01a7/366921/62a73c4d1c44/molcellb00104-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01a7/366921/62a73c4d1c44/molcellb00104-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01a7/366921/62a73c4d1c44/molcellb00104-0233-a.jpg

相似文献

1
Recombination hot spot in the human beta-globin gene cluster: meiotic recombination of human DNA fragments in Saccharomyces cerevisiae.人类β-珠蛋白基因簇中的重组热点：酿酒酵母中人类DNA片段的减数分裂重组
Mol Cell Biol. 1985 Aug;5(8):2029-38. doi: 10.1128/mcb.5.8.2029-2038.1985.
2
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引用本文的文献

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Fission yeast hotspot sequence motifs are also active in budding yeast.裂殖酵母热点序列基序在芽殖酵母中也具有活性。
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2
Nonuniform recombination within the human beta-globin gene cluster: A reply to B. S. Weir and W. G. Hill.人类β-珠蛋白基因簇内的非均匀重组：对B. S. 韦尔和W. G. 希尔的回应
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3
d(GA x TC)(n) microsatellite DNA sequences enhance homologous DNA recombination in SV40 minichromosomes.d(GA x TC)(n)微卫星DNA序列增强了SV40微型染色体中的同源DNA重组。

本文引用的文献

1
Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.人类胎儿Gγ-和Aγ-珠蛋白基因：完整的核苷酸序列表明，这些重复基因之间可发生DNA交换。
Cell. 1980 Oct;21(3):627-38. doi: 10.1016/0092-8674(80)90426-2.
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The structure of a human alpha-globin pseudogene and its relationship to alpha-globin gene duplication.人类α-珠蛋白假基因的结构及其与α-珠蛋白基因重复的关系。
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Why are some genetic diseases common? Distinguishing selection from other processes by molecular analysis of globin gene variants.为什么有些遗传疾病很常见？通过对珠蛋白基因变异进行分子分析来区分选择与其他过程。
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Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination.重组步移：通过同源重组从酵母人工染色体混合文库中进行克隆的遗传筛选。
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8118-22. doi: 10.1073/pnas.90.17.8118.
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The genomic instability associated with integrated simian virus 40 DNA is dependent on the origin of replication and early control region.与整合的猴病毒40 DNA相关的基因组不稳定性取决于复制起点和早期控制区。
J Virol. 1994 Feb;68(2):787-96. doi: 10.1128/JVI.68.2.787-796.1994.
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Hotspots of meiotic recombination in the mouse major histocompatibility complex.小鼠主要组织相容性复合体中减数分裂重组的热点区域。
Genetica. 1993;88(2-3):187-96. doi: 10.1007/BF02424475.
8
Linkage disequilibrium predicts physical distance in the adenomatous polyposis coli region.连锁不平衡可预测结肠腺瘤性息肉病区域的物理距离。
Am J Hum Genet. 1994 May;54(5):884-98.
9
Polymorphic haplotypes and recombination rates at the LDL receptor gene locus in subjects with and without familial hypercholesterolemia who are from different populations.来自不同人群的有和没有家族性高胆固醇血症的受试者中低密度脂蛋白受体基因位点的多态性单倍型和重组率。
Am J Hum Genet. 1993 Apr;52(4):808-26.
10
Recombinators, recombinases and recombination genes of yeasts.酵母的重组体、重组酶和重组基因。
Curr Genet. 1994 Jan;25(1):1-11. doi: 10.1007/BF00712959.
与酵母质粒2μm环相关的复制和重组功能。
Cell. 1980 Sep;21(2):501-8. doi: 10.1016/0092-8674(80)90487-0.
4
The DNA sequence of the 5' flanking region of the human beta-globin gene: evolutionary conservation and polymorphic differences.人类β-珠蛋白基因5'侧翼区的DNA序列：进化保守性与多态性差异
Nucleic Acids Res. 1982 Mar 25;10(6):2109-20. doi: 10.1093/nar/10.6.2109.
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Hybrid DNA formation during meiotic recombination.减数分裂重组过程中的杂种DNA形成。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7648-51. doi: 10.1073/pnas.78.12.7648.
6
Overproduction and control of the LEU2 gene product, beta-isopropylmalate dehydrogenase, in transformed yeast strains.转化酵母菌株中LEU2基因产物β-异丙基苹果酸脱氢酶的过量生产与调控
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Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast.在大肠杆菌中与β-半乳糖苷酶融合的酵母基因能够在酵母中正常表达。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2460-4. doi: 10.1073/pnas.78.4.2460.
8
A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes.一种具有形成Z-DNA潜力的新型重复元件广泛存在于进化上多样的真核生物基因组中。
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Molecular characterization of seven beta-thalassemia mutations in Asian Indians.亚洲印度人中七种β地中海贫血突变的分子特征分析
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Origin of the beta S-globin gene in blacks: the contribution of recurrent mutation or gene conversion or both.黑人中βS-珠蛋白基因的起源：反复突变或基因转换或两者的作用。
Proc Natl Acad Sci U S A. 1984 Feb;81(3):853-6. doi: 10.1073/pnas.81.3.853.