Fan Q, Xu F, Petes T D
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
Mol Cell Biol. 1995 Mar;15(3):1679-88. doi: 10.1128/MCB.15.3.1679.
The region of Saccharomyces cerevisiae chromosome III located between the 5' end of the HIS4 gene and the 3' end of the adjacent BIK1 gene has a very high level of meiotic recombination. In wild-type strains, a meiosis-specific double-strand DNA break occurs in the hot spot region. This break is absent in strains in which the transcription factors Rap1p, Bas1p, and Bas2p cannot bind to the region upstream of HIS4. In strains with levels of recombination that are higher than those of the wild type, the break is found at elevated levels. The linear relationship between hot spot activity and the frequency of double-strand DNA breaks suggests that these lesions are responsible for initiating recombination at the HIS4 recombination hot spot.
酿酒酵母第三条染色体上位于HIS4基因5'端和相邻BIK1基因3'端之间的区域具有非常高的减数分裂重组水平。在野生型菌株中，减数分裂特异性双链DNA断裂发生在热点区域。在转录因子Rap1p、Bas1p和Bas2p不能与HIS4上游区域结合的菌株中，这种断裂不存在。在重组水平高于野生型的菌株中，发现这种断裂的水平升高。热点活性与双链DNA断裂频率之间的线性关系表明，这些损伤负责在HIS4重组热点启动重组。
