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Infect Immun. 1977 Jul;17(1):73-7. doi: 10.1128/iai.17.1.73-77.1977.
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本文引用的文献

1
THE GNOTOBIOTIC DOG.无菌犬
Lab Anim Care. 1963 Aug;13:SUPPL643-9.
2
Macrophage-lymphocyte interaction. I. Characteristics of the antigen-independent-binding of guinea pig thymocytes and lymphocytes to syngeneic macrophages.巨噬细胞-淋巴细胞相互作用。I. 豚鼠胸腺细胞和淋巴细胞与同基因巨噬细胞的抗原非依赖性结合特性。
J Exp Med. 1973 Oct 1;138(4):900-24. doi: 10.1084/jem.138.4.900.
3
Receptors for complement and immunoglobulin on human and animal lymphoid cells.人和动物淋巴样细胞上的补体及免疫球蛋白受体
Transplant Rev. 1973;16:3-28. doi: 10.1111/j.1600-065x.1973.tb00115.x.
4
Receptors for aggregated IgG on mouse lymphocytes: their presence on thymocytes, thymus-derived, and bone marrow-derived lymphocytes.小鼠淋巴细胞上聚集IgG的受体：其在胸腺细胞、胸腺来源淋巴细胞及骨髓来源淋巴细胞上的存在情况。
J Exp Med. 1974 May 1;139(5):1175-88. doi: 10.1084/jem.139.5.1175.
5
Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.人类T和B淋巴细胞的表面标志物。I. 大量与绵羊红细胞形成非免疫性玫瑰花结的淋巴细胞。
J Exp Med. 1972 Aug 1;136(2):207-15. doi: 10.1084/jem.136.2.207.
6
A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization.一群带有抗原-抗体-补体复合物膜受体的淋巴细胞。I. 分离与特性鉴定。
J Exp Med. 1970 Oct 1;132(4):702-20. doi: 10.1084/jem.132.4.702.
7
Differentiating feline T and B lymphocytes by rosette formation.通过玫瑰花结形成鉴别猫的T淋巴细胞和B淋巴细胞。
J Immunol. 1975 Sep;115(3):862-5.
8
Effects of canine distemper virus infection on lymphoid function in vitro and in vivo.犬瘟热病毒感染对体内外淋巴功能的影响。
Infect Immun. 1975 May;11(5):1069-78. doi: 10.1128/iai.11.5.1069-1078.1975.
9
Rosette formation by canine peripheral blood lymphocytes.犬外周血淋巴细胞的玫瑰花结形成
J Immunol. 1975 Jan;114(1 Pt 2):399-402.
10
Spontaneous eosinophil-erythrocyte rosette formation.自发性嗜酸性粒细胞-红细胞花环形成。
Lab Invest. 1976 Sep;35(3):302-5.

犬的玫瑰花结形成试验：E 玫瑰花结对 T 淋巴细胞缺乏特异性。

Rosette formation assays in dogs: lack of specificity of E rosettes for T lymphocytes.

作者信息

Krakowka S, Guyot D J

出版信息

Infect Immun. 1977 Jul;17(1):73-7. doi: 10.1128/iai.17.1.73-77.1977.

DOI:10.1128/iai.17.1.73-77.1977
PMID:301861
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC421083/
Abstract

The present study examined the specificity of guinea pig erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosette formation assays with suspensions of canine peripheral blood lymphocytes. Neutrophils, monocytes, and lymphocytes bound EAC but not erythrocyte-antibody (EA) controls. Similarly, all three cell types formed rosettes with guinea pig E. Adherence of guinea pig E to these cells was apparently mediated by natural cytophilic antibodies present in the serum used in the suspension medium. The nonspecificity of the guinea pig E-rosette formation assay with canine lymphocytes renders the technique unreliable for the identification of thymus-derived lymphocytes in dogs.

摘要

本研究检测了豚鼠红细胞（E）和红细胞-抗体-补体（EAC）花环形成试验对犬外周血淋巴细胞悬液的特异性。中性粒细胞、单核细胞和淋巴细胞能结合EAC，但不能结合红细胞-抗体（EA）对照。同样，这三种细胞类型都能与豚鼠E形成花环。豚鼠E与这些细胞的黏附显然是由悬浮培养基中所用血清中存在的天然嗜细胞抗体介导的。豚鼠E花环形成试验对犬淋巴细胞的非特异性使得该技术在鉴定犬的胸腺来源淋巴细胞时不可靠。