Boakye Yaw D, Agyare Christian, Ayande George P, Titiloye Nicholas, Asiamah Emmanuel A, Danquah Kwabena O
Department of Pharmaceutics, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
School of Pharmacy, University of Nottingham, Nottingham, United Kingdom.
Front Pharmacol. 2018 Aug 21;9:945. doi: 10.3389/fphar.2018.00945. eCollection 2018.
(Family Euphorbiaceae) is a shrub, which is widely distributed in West Africa and employed traditionally as a wound-healing agent especially in Ghana. The aim of the study was to determine the wound-healing activity of aqueous aerial part extract of (PLE) and its major isolate, geraniin. Excision and incision wound models were used to determine the wound-healing activity. Wounds were treated with PLE (0.25, 0.5, and 1% w/w) and geraniin (0.1, 0.2, and 0.4% w/w) aqueous creams. PLE and geraniin significantly ( < 0.001) increased wound contraction rate and hydroxyproline production compared to untreated wounds. Histological studies of wound tissues showed high levels of fibroblasts and increased collagen content and cross-linking in PLE and geraniin-treated wound tissues. Immuno-histochemical investigations revealed high levels of TGF-β in PLE and geraniin-treated wound tissues compared to the untreated wound tissues. Tensile strength of incised wounds was significantly ( < 0.05) high in PLE and geraniin-treated wounds. PLE (0.1-100 μg/mL) significantly ( < 0.001) reduce LDH release from HaCaT-keratinocytes compared to the untreated cells. PLE and geraniin possess wound healing and cytoprotective effect.
（大戟科）是一种灌木，广泛分布于西非，在传统上被用作伤口愈合剂，尤其是在加纳。本研究的目的是确定（PLE）地上部分水提取物及其主要分离物老鹳草素的伤口愈合活性。采用切除和切口伤口模型来确定伤口愈合活性。伤口用PLE（0.25%、0.5%和1% w/w）和老鹳草素（0.1%、0.2%和0.4% w/w）水性乳膏进行处理。与未处理的伤口相比，PLE和老鹳草素显著（<0.001）提高了伤口收缩率和羟脯氨酸产量。伤口组织的组织学研究显示，在PLE和老鹳草素处理的伤口组织中，成纤维细胞水平较高，胶原蛋白含量和交联增加。免疫组织化学研究表明，与未处理的伤口组织相比，PLE和老鹳草素处理的伤口组织中TGF-β水平较高。在PLE和老鹳草素处理的伤口中，切口伤口的抗张强度显著（<0.05）较高。与未处理的细胞相比，PLE（0.1 - 100μg/mL）显著（<0.001）降低了HaCaT角质形成细胞中LDH的释放。PLE和老鹳草素具有伤口愈合和细胞保护作用。
