Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, South Korea.
Department of Beauty Science, Gwangju University, Gwangju, South Korea.
Phytother Res. 2018 Dec;32(12):2475-2479. doi: 10.1002/ptr.6186. Epub 2018 Sep 5.
This study aimed to investigate in vitro the anti-influenza B/Lee/40 virus effect of sakuranetin and mode of its action. The sakuranetin exhibited potent antiviral activity against influenza B/Lee/40 virus, reducing the formation of a visible cytopathic effect, with a 50% inhibitory concentration (IC ) of 7.21 μg/ml and no cytotoxicity at a concentration of 100 μg/ml, and the derived therapeutic index (TI) was >13.87. Oseltamivir showed weak anti-influenza B/Lee/40 virus activity with IC of 80.74 μg/ml, 50% cytotoxicity concentration of >100 μg/ml, and TI of >1.24. Sakuranetin also showed effective inhibitory effects when added at the viral attachment, entry, and postentry steps. Moreover, sakuranetin effectively inactivated influenza B/Lee/40 virus infection in dose- and temperature-dependent manners. Sakuranetin indicated an inhibitory effect in viral RNA synthesis in the presence of 100 μg/ml of sakuranetin. Overall, this research revealed that sakuranetin could inhibit influenza B/Lee/40 virus replication and that sakuranetin may be involved in the virus attachment, entry, and postentry. Therefore, sakuranetin is a good candidate for a chemopreventive agent for influenza virus-related diseases.
本研究旨在体外研究樱黄素抗乙型流感/Lee/40 病毒的作用及其作用模式。樱黄素对乙型流感/Lee/40 病毒表现出强大的抗病毒活性，可减少可见细胞病变效应的形成，其 50%抑制浓度（IC ）为 7.21μg/ml，在 100μg/ml 浓度下无细胞毒性，治疗指数（TI）大于 13.87。奥司他韦对乙型流感/Lee/40 病毒的活性较弱，IC 为 80.74μg/ml，50%细胞毒性浓度大于 100μg/ml，TI 大于 1.24。樱黄素在病毒附着、进入和进入后阶段添加时也表现出有效的抑制作用。此外，樱黄素以剂量和温度依赖的方式有效灭活乙型流感/Lee/40 病毒感染。在存在 100μg/ml 樱黄素的情况下，樱黄素显示出对病毒 RNA 合成的抑制作用。总体而言，本研究表明樱黄素可以抑制乙型流感/Lee/40 病毒的复制，樱黄素可能参与病毒的附着、进入和进入后阶段。因此，樱黄素是一种预防流感病毒相关疾病的候选药物。
