Department of Environmental Science and Engineering, School of Space and Environment , Beihang University , Beijing 100191 , China.
Graduate School of Agricultural & Life Sciences , The University of Tokyo , Yayoi 1-1-1 , Bunkyo-ku, Tokyo 113-8657 , Japan.
Langmuir. 2018 Oct 2;34(39):11878-11885. doi: 10.1021/acs.langmuir.8b02440. Epub 2018 Sep 18.
Hydrogen water as a new therapeutic antioxidant has been widely used in living organisms under stress. In this study, we applied nanobubble (NB) technology to hydrogen water. The antioxidant capacity of hydrogen NB water was studied with respect to different reactive oxygen species (ROS) both in vitro and in vivo. Using a relatively weak reduced dye, APF, we showed that hydrogen NB water can effectively remove three cytotoxic ROS, OH, ClO, and ONOO, from water. Hydrogen NB water could also remove O, which is a physiologically important ROS, from water. However, hydrogen water could not reduce other physiologically important ROS such as HO and NO. At similar dissolved hydrogen concentrations, hydrogen NB water displayed higher antioxidant activity than hydrogen water without NB. Barley seed germination tests were used to study the antioxidant effect of hydrogen NB water on ROS generation in vivo. Our results showed that this decreased the physiological activity of barley seeds in their normal homeostatic state. Hydrogen NB water eliminated endogenous O in seeds and inhibited germination. The usage of hydrogen NB water should be individually considered according to the types of cells involved. Our results offer basic data concerning the application of hydrogen NB water in different fields.
氢气水作为一种新型的治疗性抗氧化剂，在应激状态下的生物体中得到了广泛的应用。在本研究中，我们将纳米气泡（NB）技术应用于氢气水中。我们研究了氢气 NB 水对不同活性氧（ROS）的抗氧化能力，包括体外和体内实验。使用一种相对较弱的还原染料，APF，我们表明氢气 NB 水可以有效地从水中去除三种细胞毒性 ROS，OH、ClO 和 ONOO。氢气 NB 水还可以从水中去除 O，O 是一种重要的生理 ROS。然而，氢气水不能还原其他重要的生理 ROS，如 HO 和 NO。在相似的溶解氢浓度下，氢气 NB 水显示出比不含 NB 的氢气水更高的抗氧化活性。大麦种子发芽试验用于研究氢气 NB 水在体内对 ROS 生成的抗氧化作用。我们的结果表明，这降低了大麦种子在正常自稳状态下的生理活性。氢气 NB 水消除了种子中的内源性 O，并抑制了发芽。氢气 NB 水的使用应根据涉及的细胞类型进行单独考虑。我们的研究结果为氢气 NB 水在不同领域的应用提供了基础数据。
