Hemmi S, Fenner M, Gall E, Binz H, Wigzell H
Scand J Immunol. 1986 Mar;23(3):327-49. doi: 10.1111/j.1365-3083.1986.tb01974.x.
Polyclonal syngeneic, allogeneic, and xenogeneic and monoclonal syngeneic anti-anti-idiotypic antibodies have been produced against previously described monoclonal anti-idiotypic antibodies with specificity for monoclonal RT1 alloantigen-specific antibodies. The anti-anti-idiotypes could again be shown to be highly specific for the monoclonal anti-idiotype used for the induction of the anti-anti-idiotypic antibodies and to carry the same, or a very similar, idiotype as the original monoclonal idiotypic antibody used to induce the monoclonal anti-idiotypic. Among the 30 syngeneic and allogeneic and the five xenogeneic polyclonal anti-anti-idiotypic antisera and the three monoclonal anti-anti-idiotypes, only one polyclonal antiserum showed binding capacity to the corresponding RT1-encoded antigenic determinants on spleen cells. All the other antibodies were idiotypic but not antigen binding.
已针对先前描述的对单克隆RT1同种异体抗原特异性抗体具有特异性的单克隆抗独特型抗体，制备了多克隆同基因、异基因和异种以及单克隆同基因抗抗独特型抗体。抗抗独特型抗体再次被证明对用于诱导抗抗独特型抗体的单克隆抗独特型具有高度特异性，并携带与用于诱导单克隆抗独特型的原始单克隆独特型抗体相同或非常相似的独特型。在30种同基因和异基因以及5种异种多克隆抗抗独特型抗血清和3种单克隆抗抗独特型中，只有一种多克隆抗血清显示出与脾细胞上相应RT1编码的抗原决定簇的结合能力。所有其他抗体都是独特型的，但不具有抗原结合能力。
