Hu L F, Wang L P, Ju L W, Huang L H, Li X L, Li X Z, Gu J R
Sci Sin B. 1986 Feb;29(2):181-6.
Poly(A)+ RNA was isolated from 9 specimens of human primary hepatic carcinoma, 1 non-tumorous liver tissue adjacent to cancer and 1 normal liver tissue samples. The Oligo-dT cellulose-purified poly(A)+ RNAs were subjected to formaldehyde agarose gel electrophoresis, Northern transfer and hybridization with various oncogene probes. Two RNA species, 5.6 kb and 2.2 kb were identified by N-ras gene hybridization in 6 out of 9 mRNA samples from primary hepatic carcinoma specimen. N-ras specific mRNA was not detectable in mRNA samples from normal human liver and tumor surrounding cirrhotic tissue. No detectable hybridization of mRNA from hepatoma and normal liver with Ki-ras or Ha-ras was observed. As human N-ras gene has been identified in DNA of mouse transfectants transformed with PHC DNA, it strongly suggests that N-ras gene might be responsible for the transforming activity of part of cases of human liver cancer.
从9份人类原发性肝癌标本、1份癌旁非肿瘤肝组织及1份正常肝组织样本中分离出Poly(A)+ RNA。将经寡聚dT纤维素纯化的Poly(A)+ RNA进行甲醛琼脂糖凝胶电泳、Northern转移，并与各种癌基因探针杂交。在9份原发性肝癌标本的mRNA样本中，有6份通过N-ras基因杂交鉴定出两种RNA条带，分别为5.6 kb和2.2 kb。在正常人肝和肿瘤周围肝硬化组织的mRNA样本中未检测到N-ras特异性mRNA。未观察到肝癌和正常肝的mRNA与Ki-ras或Ha-ras有可检测到的杂交。由于在用人原发性肝癌DNA转化的小鼠转染子的DNA中已鉴定出人类N-ras基因，这有力地表明N-ras基因可能是部分人类肝癌病例转化活性的原因。
