Bu H
West China University of Medical Sciences, Chengdu.
Zhonghua Bing Li Xue Za Zhi. 1991 Mar;20(1):11-3.
30 cases of hepatocellular carcinoma and pericancerous nontumor samples were studied by examining the serial sections for immunohistochemical detection of P21 product accompanying identification of N-ras mRNA by in situ hybridization with biotinylated N-ras cDNA probe and a significant correlation was noticed between the results obtained (P less than 0.02). It was suggested that immunohistochemical detection of ras oncogene P21 product may indicate the expression of ras oncogene. The higher expression of N-ras mRNA of the carcinoma and the non-tumor tissue peripheral to the cancer by in situ hybridization were 53.33% and 28.57% respectively. The positive ratio of P21 were 96.67% and 90.48%.
对30例肝细胞癌及癌旁非肿瘤组织样本进行研究，通过连续切片进行免疫组织化学检测P21产物，同时用生物素化的N-ras cDNA探针原位杂交鉴定N-ras mRNA，结果发现两者之间存在显著相关性（P<0.02）。提示ras癌基因P21产物的免疫组织化学检测可能反映ras癌基因的表达。原位杂交显示癌组织及癌旁非肿瘤组织中N-ras mRNA的高表达率分别为53.33%和28.57%。P21的阳性率分别为96.67%和90.48%。
