Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland.
Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland.
Int J Food Microbiol. 2019 Jan 16;289:1-6. doi: 10.1016/j.ijfoodmicro.2018.08.029. Epub 2018 Aug 31.
The aim of the study was to determine antimicrobial resistance and genotypic characteristics of L. monocytogenes isolated from food of animal origin from different parts of Poland during years 2013-2016. A total of 146 isolates were tested using a microbroth dilution method, whereas virulence genes and molecular serogroups were identified by PCR. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) methods were used to analyze the genotypic relationship of the strains. Altogether, 102 pulsotypes grouped into 7 clusters and 24 sequence types, including 3 new types, were identified. Most of the strains clustered into individual patterns were originated from different food products and were isolated in different geographical regions at various time. L. monocytogenes was mostly resistant to oxacilin (90.4% strains), clindamycin (54.1%) and ceftriaxone (49.3%). A multiresistance patterns, mainly to ceftriaxone, oxacillin together with other antimicrobials, were observed among 27.4% strains. Antimicrobial resistance and presence of virulence genes suggest that food of animal origin contaminated with L. monocytogenes may present a risk for public health.
本研究旨在确定 2013 年至 2016 年期间波兰不同地区动物源食品中分离的单核细胞增生李斯特菌的抗菌药物耐药性和基因型特征。使用微量肉汤稀释法对 146 株分离株进行了测试，而通过 PCR 鉴定了毒力基因和分子血清群。脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）方法用于分析菌株的基因型关系。总共鉴定出 102 个脉冲场凝胶电泳型，分为 7 个聚类和 24 个序列型，包括 3 种新型。大多数聚类为单个模式的菌株来自不同的食品，并且在不同的地理区域和不同的时间进行了分离。单核细胞增生李斯特菌对苯唑西林（90.4%的菌株）、克林霉素（54.1%）和头孢曲松（49.3%）的耐药率最高。27.4%的菌株表现出主要对头孢曲松、苯唑西林以及其他抗菌药物的多耐药模式。抗菌药物耐药性和毒力基因的存在表明，受单核细胞增生李斯特菌污染的动物源食品可能对公众健康构成威胁。
