Jashari Besart, Stessl Beatrix, Félix Benjamin, Cana Armend, Bisha Bledar, Jankuloski Dean, Blagoevska Katerina, Kayode Adeoye J
Food Microbiology, Food and Veterinary Laboratory, Food and Veterinary Agency of Kosovo, Lidhja e Pejës 241, 10000 Pristina, Kosovo.
Unit of Food Microbiology, Centre for Food Science and Public Veterinary Health, Clinical Department for Farm Animals and Food Systems Safety, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-2110 Vienna, Austria.
Microorganisms. 2024 Nov 27;12(12):2441. doi: 10.3390/microorganisms12122441.
In the absence of data on the reporting of resistance to antibiotics, we sought to determine which clonal complexes (CCs)/sequence types (STs) circulate in the food chain in Kosovo and to determine their antibiogram profiles to a panel of 18 antibiotics. From a total of 114 isolates, 21 different typical STs were identified by multilocus sequence typing (MLST). Each isolate derived from the food categories was subjected to tests to verify its susceptibility to the selected antibiotics according to the designed Sensititre GPN3F panel. Among the different STs that were identified, CC9-ST9 was more abundant in meat products (38.75%) while CC29-ST29 was more abundant (24.0%) in dairy products. Moreover, these isolates showed marked resistance against levofloxacin (22.8%), gentamicin and rifampicin (17.5%), quinupristin/dalfopristin (14.9%), erythromycin (11.4%), penicillin (7.89%), tetracycline (1.75%), and streptomycin (0.88%). A total of 27 multiple antibiotic resistance (MAR) phenotypes were observed amongst the isolates, which ranged from 3 to 12. The ARI of the food category including meat and meat products (MMP, 0.22) and fish meat products (FMP, 0.26) were >0.2, the permissible Krumperman threshold. The number of strains with MAR values >0.2 was 34, (29.8%). The identification of typical multidrug-resistant STs among isolates in Kosovo constitutes a potential threat to food safety and public health, which requires a continuous and expanded surveillance system to prevent the further spread of antimicrobial resistant (AMR) isolates.
在缺乏抗生素耐药性报告数据的情况下，我们试图确定科索沃食物链中传播的哪些克隆复合体（CCs）/序列类型（STs），并确定它们对一组18种抗生素的抗菌谱。通过多位点序列分型（MLST）从总共114株分离物中鉴定出21种不同的典型STs。根据设计的Sensititre GPN3F平板，对来自各类食品的每株分离物进行测试，以验证其对所选抗生素的敏感性。在鉴定出的不同STs中，CC9-ST9在肉类产品中更为常见（38.75%），而CC29-ST29在乳制品中更为常见（24.0%）。此外，这些分离物对左氧氟沙星（22.8%）、庆大霉素和利福平（17.5%）、奎奴普丁/达福普汀（14.9%）、红霉素（11.4%）、青霉素（7.89%）、四环素（1.75%）和链霉素（0.88%）表现出明显耐药性。在分离物中总共观察到27种多重抗生素耐药（MAR）表型，范围从3到12。包括肉类和肉类产品（MMP，0.22）以及鱼肉产品（FMP，0.26）在内的食品类别抗生素抗性指数（ARI）>0.2，即克鲁珀曼允许阈值。MAR值>0.2的菌株数量为34株（29.8%）。在科索沃分离物中鉴定出典型的多重耐药STs对食品安全和公众健康构成潜在威胁，这需要一个持续且扩大的监测系统来防止抗菌药物耐药（AMR）分离物的进一步传播。
