单核细胞增生李斯特菌、无害李斯特菌和威尔斯李斯特菌的抗菌药物敏感性比较。
Comparative antimicrobial susceptibility of Listeria monocytogenes, L. innocua, and L. welshimeri.
机构信息
Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Richard B. Russell Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA.
出版信息
Microb Drug Resist. 2009 Mar;15(1):27-32. doi: 10.1089/mdr.2009.0863.
The current study compared antimicrobial susceptibility of Listeria innocua, L. welshimeri, and L. monocytogenes isolated from various sources. Antimicrobial susceptibility testing was performed using a microbroth procedure with Sensititre minimum inhibitory concentration plates containing 18 antimicrobials. Resistant isolates were analyzed for the presence of antimicrobial resistance genes using PCR. The majority of L. monocytogenes isolates were resistant to oxacillin (99%, 89/90) and ceftriaxone (72%, 65/90), while few isolates were resistant to clindamycin (21%, 19/90) and ciprofloxacin (2%, 2/90). When selected sources of L. monocytogenes are compared, resistance to ceftriaxone, clindamycin, and oxacillin ranged from 27% to 86%, 7% to 43%, and 96% to 100%, respectively. Resistance to ciprofloxacin (6%, 2/34), quinupristin/dalfopristin (7%, 1/14), and tetracycline (7%, 1/15) was observed with L. monocytogenes isolated from food, animal, and environmental sources, respectively. All L. welshimeri isolates (6/6) were resistant to streptomycin, quinupristin/dalfopristin, ciprofloxacin, rifampin, oxacillin, penicillin, and clindamycin, while most isolates (67%, 4/6) were resistant to trimethoprim/sulfamethoxazole. All L. innocua isolates (4/4) were resistant to oxacillin and penicillin, whereas 75% (3/4) of isolates were resistant to tetracycline, ceftriaxone, and clindamycin. Resistant isolates were negative for aadA, strA-B, sul I-II, penA, vat(A-E), vga(A-B), and vgb(A-B). However, tetM was detected among tetracycline-resistant isolates. L. welshimeri was resistant to more of the tested antimicrobials than the other two Listeria species tested, but resistance was not attributed to selected resistance genes. These data demonstrate the variability in resistance among Listeria species. However, the human pathogen L. monocytogenes appears to be the least resistant among the tested species.
本研究比较了来源于不同来源的无害李斯特菌、威氏李斯特菌和单增李斯特菌的抗菌药物敏感性。抗菌药物敏感性测试采用微量肉汤法，使用 Sensititre 最低抑菌浓度平板，其中包含 18 种抗菌药物。使用 PCR 分析耐药分离株中是否存在抗菌药物耐药基因。大多数单增李斯特菌分离株对苯唑西林（99%，89/90）和头孢曲松（72%，65/90）耐药，而少数分离株对克林霉素（21%，19/90）和环丙沙星（2%，2/90）耐药。当比较单增李斯特菌的选定来源时，头孢曲松、克林霉素和苯唑西林的耐药率分别为 27%至 86%、7%至 43%和 96%至 100%。在食品、动物和环境来源的单增李斯特菌中分别观察到对环丙沙星（6%，2/34）、奎奴普丁/达福普汀（7%，1/14）和四环素（7%，1/15）的耐药性。所有威氏李斯特菌分离株（6/6）均对链霉素、奎奴普丁/达福普汀、环丙沙星、利福平、苯唑西林、青霉素和克林霉素耐药，而大多数分离株（67%，4/6）对甲氧苄啶/磺胺甲噁唑耐药。所有无害李斯特菌分离株（4/4）均对苯唑西林和青霉素耐药，而 75%（3/4）的分离株对四环素、头孢曲松和克林霉素耐药。耐药分离株对 aadA、strA-B、sul I-II、penA、vat(A-E)、vga(A-B) 和 vgb(A-B) 均为阴性。然而，在四环素耐药分离株中检测到 tetM。威氏李斯特菌对测试的抗菌药物比其他两种测试的李斯特菌更耐药，但耐药性并非归因于选定的耐药基因。这些数据表明李斯特菌属种间耐药性存在差异。然而，在测试的物种中，人类病原体单增李斯特菌似乎耐药性最低。
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