Department of Musculoskeletal and Plastic Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Department of Surgery, Päijät-Häme Central Hospital, Lahti, Finland.
J Biomed Mater Res B Appl Biomater. 2019 Apr;107(3):847-857. doi: 10.1002/jbm.b.34181. Epub 2018 Sep 8.
The two-stage induced-membrane (IM) technique is increasingly used for treatment of large bone defects. In stage one, the bone defect is filled with polymethylmethacrylate (PMMA), which induces a membrane around the implant. In stage two, PMMA is replaced with bone graft. Bioactive glasses (BAGs) are bone substitutes with bone-stimulating and angiogenic properties. We have previously shown that a certain type of BAG can also induce a foreign-body membrane similar to PMMA. The aim of this study was to evaluate the bone-forming capacity of sintered BAG-S53P4 and poly(lactide-co-glycolide) (PLGA)-coated BAG-S53P4 scaffolds for potential use as bone substitutes in a single-stage IM technique. Sintered porous rods of BAG-S53P4, BAG-S53P4-PLGA, or PMMA were implanted in rabbit femurs for 2, 4, or 8 weeks. The expression of bone morphogenic protein (BMP)-2, -4, and -7 in the IMs of implanted materials were analyzed with real-time quantitative polymerase chain reaction. Micro-computed tomography imaging was used to evaluate bone growth and further verified with scanning electron microscopy. BAG-S53P4 and BAG-S53P4-PGLA scaffold IMs show similar or superior expression of BMP-2, -4, and -7 compared with PMMA IM. Bone ingrowth into BAG scaffolds increased over time. Active bone formation occurred inside the BAG scaffolds and the respective BMP expressions were similar or superior for the BAG IMs compared with PMMA, thus making BAGs a promising device for single-stage treatment of bone defects. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res B Part B: Appl Biomater, 2018. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 847-857, 2019.
两阶段诱导膜（IM）技术越来越多地用于治疗大的骨缺损。在第一阶段，用聚甲基丙烯酸甲酯（PMMA）填充骨缺损，在植入物周围诱导形成膜。在第二阶段，PMMA 被骨移植物取代。生物活性玻璃（BAG）是一种具有骨刺激和血管生成特性的骨替代物。我们之前已经表明，某种类型的 BAG 也可以诱导类似于 PMMA 的异物膜。本研究旨在评估烧结 BAG-S53P4 和聚（乳酸-共-乙醇酸）（PLGA）涂层 BAG-S53P4 支架的成骨能力，以潜在地用作单相 IM 技术中的骨替代物。将烧结的 BAG-S53P4、BAG-S53P4-PLGA 或 PMMA 多孔棒植入兔股骨 2、4 或 8 周。通过实时定量聚合酶链反应分析植入材料的诱导膜中骨形态发生蛋白（BMP）-2、-4 和 -7 的表达。使用微计算机断层扫描成像评估骨生长，并进一步通过扫描电子显微镜验证。与 PMMA 相比，BAG-S53P4 和 BAG-S53P4-PLGA 支架 IM 的 BMP-2、-4 和 -7 表达相似或更高。随着时间的推移，BAG 支架内的骨向内生长增加。BAG 支架内发生活跃的骨形成，BAG IM 的 BMP 表达与 PMMA 相似或更高，因此 BAG 成为治疗骨缺损的单相治疗的有前途的装置。©2018 威利父子公司。生物材料研究杂志 B 部分：应用生物材料，2018.©2018 威利父子公司。生物材料研究杂志 B 部分：应用生物材料 107B：847-857，2019.
