Kleiner E, Dietrich W, Pfister H
EMBO J. 1986 Aug;5(8):1945-50. doi: 10.1002/j.1460-2075.1986.tb04448.x.
Treatment of bovine papilloma virus (BPV) 1-transformed mouse fibroblasts with cycloheximide led to a 10-fold increase in the amount of viral transcripts, after as little as 1 h of protein synthesis inhibition. Northern blots revealed no qualitative changes in the RNA pattern. Nuclear run-on experiments showed about a 7-fold increase in specific transcriptional activity after cycloheximide treatment. The half-life of BPV1 mRNA was twice as long as in untreated controls. These results indicate that both RNA synthesis and degradation of viral RNA are controlled by labile proteins. Cycloheximide stimulation turned out to be independent of the BPV1 E2 gene activity which enhances viral transcription. Cycloheximide treatment had no effect on the amount of human papilloma virus (HPV) 18 transcripts in cervical carcinoma derived HeLa and C4-1 cells. Transcription of HPV16 in the cervical carcinoma line SiHa was likewise unaffected. The differential regulation of transcription in transformed fibroblasts and cancer-derived cells, and the significance for malignant conversion are discussed.
用环己酰亚胺处理牛乳头瘤病毒（BPV）1转化的小鼠成纤维细胞，在蛋白质合成抑制仅1小时后，病毒转录本的量就增加了10倍。Northern印迹显示RNA模式没有定性变化。核转录实验表明，环己酰亚胺处理后特异性转录活性增加了约7倍。BPV1 mRNA的半衰期是未处理对照的两倍。这些结果表明，病毒RNA的合成和降解均受不稳定蛋白质的控制。结果表明，环己酰亚胺刺激与增强病毒转录的BPV1 E2基因活性无关。环己酰亚胺处理对宫颈癌来源的HeLa和C4-1细胞中人乳头瘤病毒（HPV）18转录本的量没有影响。宫颈癌系SiHa中HPV16的转录同样不受影响。本文讨论了转化成纤维细胞和癌源细胞中转录的差异调节及其对恶性转化的意义。
