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通过热解柠檬酸钠制备超小单层石墨烯量子点用于荧光细胞成像。

Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging.

机构信息

Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, People's Republic of China.

School of Public Health, Xiamen University, Xiamen 361102, People's Republic of China.

出版信息

Int J Nanomedicine. 2018 Aug 24;13:4807-4815. doi: 10.2147/IJN.S168570. eCollection 2018.

DOI:10.2147/IJN.S168570
PMID:30197516
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6113908/
Abstract

BACKGROUND

The preparation and biological applications of ultra-small graphene quantum dots (GQDs) with accurate-controlled size are of great significance.

METHODS

Here in, we report a novel procedure involving pyrolysis of trisodium citrate and subsequent ultrafiltration for fabricating monolayer GQDs with ultra-small lateral size (1.3±0.5 nm).

RESULTS

The GQDs exhibit blue photoluminescence with peak position independent of excitation wavelength. The quantum yield of GQDs is measured to be 3.6%, and the average fluorescence lifetime is 2.78 ns.

CONCLUSION

Because of high stability and low toxicity, GQDs are demonstrated to be excellent bioimaging agents. The ultra-small GQDs can not only distribute in the cytoplasm but also penetrate into the nuclei. We ensure that this work will add a new dimension to the application of graphene materials for nanomedicine.

摘要

背景

具有精确控制尺寸的超小石墨烯量子点（GQDs）的制备和生物应用具有重要意义。

方法

在这里，我们报告了一种新的方法，涉及柠檬酸三钠的热解和随后的超滤，以制造具有超小横向尺寸（1.3±0.5nm）的单层 GQDs。

结果

GQDs 表现出与激发波长无关的蓝色光致发光峰位置。GQDs 的量子产率测量为 3.6%，平均荧光寿命为 2.78ns。

结论

由于高稳定性和低毒性，GQDs 被证明是优秀的生物成像剂。超小 GQDs 不仅可以分布在细胞质中，还可以穿透到细胞核中。我们确保这项工作将为纳米医学中石墨烯材料的应用增添新的维度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/3479a6b45ade/ijn-13-4807Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/cdacfc248bb6/ijn-13-4807Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/ca16c725e6ad/ijn-13-4807Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/f71be7617f67/ijn-13-4807Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/5bb2aee2f0c5/ijn-13-4807Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/b05646e0f9b0/ijn-13-4807Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/53d317ae3b26/ijn-13-4807Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/3479a6b45ade/ijn-13-4807Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/cdacfc248bb6/ijn-13-4807Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/ca16c725e6ad/ijn-13-4807Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/f71be7617f67/ijn-13-4807Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/5bb2aee2f0c5/ijn-13-4807Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/b05646e0f9b0/ijn-13-4807Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/53d317ae3b26/ijn-13-4807Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca2/6113908/3479a6b45ade/ijn-13-4807Fig7.jpg

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