Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland.
Department of Biophysics, Institute of Materials Science, Lodz University of Technology, Lodz, Poland.
J Biomed Mater Res B Appl Biomater. 2019 May;107(4):1024-1034. doi: 10.1002/jbm.b.34195. Epub 2018 Sep 10.
Today, the extensive and constantly growing number of applications in the field of nanotechnology poses a lot of questions about the potential toxicity of nanomaterials (NMs) toward cells of different origins. In our work we employed the tools of molecular biology to evaluate changes that occur in human endothelial cells at the transcriptomic and proteomic level, following 24 h of exposure to three different classes of NMs. Using microarray technology, we demonstrated that 24 h of exposure to silver nanoparticles (SNPs), multiwalled carbon nanotubes (MWCNTs) and polyamidoamine dendrimers (PAMAMs) leads to changes in 299, 1271, and 431 genes, respectively, influencing specific molecular pathways. The 2D-DIGE and mass spectrometry analysis revealed that differentially expressed proteins were involved in numerous cellular processes, for example, cytoskeletal reorganization, cell growth and proliferation, or response to stress. Both, transcriptome and proteome alterations indicate reorganization of mechanism regulating cell functioning. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1024-1034, 2019.
如今，纳米技术领域中广泛且不断增长的应用对纳米材料（NMs）对不同来源细胞的潜在毒性提出了很多问题。在我们的工作中，我们采用了分子生物学的工具，在暴露于三种不同类型的纳米材料 24 小时后，在转录组和蛋白质组水平上评估了人内皮细胞发生的变化。使用微阵列技术，我们证明暴露于银纳米颗粒（SNPs）、多壁碳纳米管（MWCNTs）和聚酰胺-胺树枝状大分子（PAMAMs）24 小时分别导致 299、1271 和 431 个基因的变化，影响特定的分子途径。2D-DIGE 和质谱分析显示，差异表达的蛋白质参与了许多细胞过程，例如细胞骨架重组、细胞生长和增殖，或对压力的反应。转录组和蛋白质组的改变都表明调节细胞功能的机制发生了重组。©2018 年 Wiley 期刊，生物医学材料研究杂志 B 部分：应用生物材料 107B:1024-1034，2019 年。
