Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Gene. 2019 Aug 20;710:210-217. doi: 10.1016/j.gene.2019.06.006. Epub 2019 Jun 7.
Low temperature is a key stress factor for the growth and development of wheat (Triticum aestivum L.), and glycometabolism plays an important role in plant cold tolerance. Our previous study identified trehalose 6-phosphate synthase 11 gene (TaTPS11), which had a significantly different expression pattern between a high freezing-tolerant wheat cultivar and a low freezing-tolerant wheat cultivar. In this study, TaTPS11 was isolated from a winter-hardy wheat cultivar (D1) and overexpressed in Arabidopsis thaliana to study its effect on cold tolerance in plants. Transgenic plants expressing TaTPS11 had lower sucrose content, higher starch content, and higher activity of key enzyme (sucrose phosphate synthase, sucrose synthase, and invertase) involved in sucrose metabolism. In addition, the expression level of sucrose non-fermenting 1-related kinase 1 (SnRK1), which catalyzes the sucrose in plants, increased in the TaTPS11-overexpressed plants. These results indicated that heterologous expression of TaTPS11 influenced carbohydrate metabolism in Arabidopsis plants. The resultant plants had a significantly higher survival rate after -5 °C treatment for 2 h and exhibited enhanced cold tolerance without unfavorable phenotypes compared to wild-type. Our findings indicated that manipulation of TaTPS11 improved cold tolerance in plants and TaTPS11 had potential values in wheat cold-tolerance breeding.
低温是小麦（Triticum aestivum L.）生长发育的关键胁迫因素，糖代谢在植物的耐寒性中起着重要作用。我们之前的研究鉴定了海藻糖-6-磷酸合酶 11 基因（TaTPS11），它在高抗寒小麦品种和低抗寒小麦品种之间的表达模式有明显的差异。在这项研究中，从冬小麦品种（D1）中分离出 TaTPS11，并在拟南芥中过表达，以研究其对植物耐寒性的影响。过表达 TaTPS11 的转基因植物的蔗糖含量较低，淀粉含量较高，参与蔗糖代谢的关键酶（蔗糖磷酸合酶、蔗糖合酶和转化酶）活性较高。此外，催化植物中蔗糖的蔗糖非发酵 1 相关激酶 1（SnRK1）的表达水平在过表达 TaTPS11 的植物中增加。这些结果表明，TaTPS11 的异源表达影响了拟南芥植物的碳水化合物代谢。与野生型相比，经过-5°C处理 2 小时后，这些植物的存活率有显著提高，表现出增强的耐寒性，而没有不良表型。我们的研究结果表明，操纵 TaTPS11 可以提高植物的耐寒性，TaTPS11 在小麦耐寒性育种中具有潜在的应用价值。
