Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangdong Engineering Technology Research Center of Nutrition Translation, Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.
Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Molecules. 2018 Aug 31;23(9):2216. doi: 10.3390/molecules23092216.
The consumption of herbal teas has become popular in recent years due to their attractive flavors and outstanding antioxidant properties. The Five-Golden-Flowers tea is a herbal tea consisting of five famous edible flowers. The effects of microwave-assisted extraction parameters on the antioxidant activity of Five-Golden-Flowers tea were studied by single-factor experiments, and further investigated using response surface methodology. Under the optimal parameters (53.04 mL/g of solvent/material ratio, 65.52 °C, 30.89 min, and 500 W), the ferric-reducing antioxidant power, Trolox equivalent antioxidant capacity, and total phenolic content of the herbal tea were 862.90 ± 2.44 µmol Fe/g dry weight (DW), 474.37 ± 1.92 µmol Trolox/g DW, and 65.50 ± 1.26 mg gallic acid equivalent (GAE)/g DW, respectively. The in vivo antioxidant activity of the herbal tea was evaluated on alcohol-induced acute liver injury in mice. The herbal tea significantly decreased the levels of aspartate aminotransferase, total bilirubin, and malonaldehyde at different doses (200, 400, and 800 mg/kg); improved the levels of liver index, serum triacylglycerol, and catalase at dose of 800 mg/kg. These results indicated its role in alleviating hepatic oxidative injury. Besides, rutin, chlorogenic acid, epicatechin, gallic acid, and -coumaric acid were identified and quantified by high performance liquid chromatography (HPLC), which could contribute to the antioxidant activity of the herbal tea.
近年来，由于其诱人的风味和出色的抗氧化特性，草本茶的消费变得流行起来。五花茶是一种由五种著名的可食用花卉组成的草本茶。通过单因素实验研究了微波辅助提取参数对五花茶抗氧化活性的影响，并进一步采用响应面法进行了研究。在最佳参数（溶剂/物料比 53.04 mL/g、65.52°C、30.89 min 和 500 W）下，该草本茶的铁还原抗氧化能力、Trolox 当量抗氧化能力和总酚含量分别为 862.90±2.44 µmol Fe/g 干重（DW）、474.37±1.92 µmol Trolox/g DW 和 65.50±1.26 mg 没食子酸当量（GAE）/g DW。该草本茶的体内抗氧化活性在小鼠急性酒精性肝损伤模型中进行了评价。该草本茶在不同剂量（200、400 和 800 mg/kg）下显著降低了天冬氨酸转氨酶、总胆红素和丙二醛的水平；在 800 mg/kg 剂量下提高了肝指数、血清三酰甘油和过氧化氢酶的水平。这些结果表明其在缓解肝氧化损伤方面的作用。此外，通过高效液相色谱（HPLC）鉴定和定量了芦丁、绿原酸、表儿茶素、没食子酸和 -咖啡酸，它们可能有助于该草本茶的抗氧化活性。
