Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland.
Department of Electronics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Krakow, Poland.
Sensors (Basel). 2018 Sep 2;18(9):2912. doi: 10.3390/s18092912.
In designing a bacteria biosensor, various issues must be addressed: the specificity of bacteria recognition, the immobilization of biomolecules that act as the bacteria receptor, and the selectivity of sensor surface. The aim of this paper was to examine how the biofunctionalized surface of Ti, Au, and Ru metals reacts in contact with strains of (. ) The focus on metal surfaces results from their future use as electrodes in high frequency biosensors, e.g., resonant circuits or transmission-line sections. First, the surfaces of different metals were chemically functionalized with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde or with 3-glycidylooxypropyltrimethoxysilane (GPTMS) followed by -(5-amino-1-carboxypentyl) iminodiacetic acid (AB-NTA) and NiCl₂. Secondly, the lipopolysaccharide binding protein (LBP), polyclonal anti- antibody and bacteriophage protein gp37 were tested as bacteria receptors. The selectivity and specificity have been confirmed by the Enzyme-Linked Immunosorbent Assay (ELISA) and visualized by scanning electron microscopy at low landing energies. We noticed that LBP, polyclonal antibody, and gp37 were successfully immobilized on all studied metals and recognized the . bacteria selectively. However, for the antibody, the highest reactivity was observed when Ti surface was modified, whereas the bacteria binding was comparable between LBP and gp37 on the functionalized Ru surfaces, independent from modification. Thus, all surfaces were biocompatible within the scope of biosensor functionality, with titanium functionalization showing the best performance.
在设计细菌生物传感器时，必须解决各种问题：细菌识别的特异性、作为细菌受体的生物分子的固定化以及传感器表面的选择性。本文旨在研究 Ti、Au 和 Ru 金属的生物功能化表面在与 (. ) 菌株接触时的反应。关注金属表面是因为它们未来将作为高频生物传感器（例如谐振电路或传输线部分）的电极使用。首先，用 3-氨丙基三乙氧基硅烷 (APTES) 和戊二醛或 3-缩水甘油氧基丙基三甲氧基硅烷 (GPTMS) 对不同金属的表面进行化学功能化，然后用 -(5-氨基-1-羧基戊基)亚氨基二乙酸 (AB-NTA) 和 NiCl₂进行修饰。其次，测试了脂多糖结合蛋白 (LBP)、多克隆抗-抗体和噬菌体蛋白 gp37 作为细菌受体。通过酶联免疫吸附试验 (ELISA) 证实了选择性和特异性，并通过低着陆能扫描电子显微镜进行了可视化观察。我们注意到 LBP、多克隆抗体和 gp37 成功地固定在所有研究的金属上，并特异性地识别了 (. ) 细菌。然而，对于抗体，当 Ti 表面进行修饰时，观察到最高的反应性，而在功能化 Ru 表面上，LBP 和 gp37 的细菌结合是可比的，而与修饰无关。因此，所有表面在生物传感器功能范围内都是生物相容的，其中钛的功能化表现出最佳性能。
