TORC1-Nem1/Spo7-Pah1/lipin 轴调控芽殖酵母中微自噬的诱导。
The TORC1-Nem1/Spo7-Pah1/lipin axis regulates microautophagy induction in budding yeast.
机构信息
Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
Department of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
出版信息
Biochem Biophys Res Commun. 2018 Oct 2;504(2):505-512. doi: 10.1016/j.bbrc.2018.09.011. Epub 2018 Sep 7.
Nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1) protein kinase promotes macroautophagy. Macroautophagy is a lipid-consuming process, and Nem1/Spo7 protein phosphatase and Pah1/lipin phosphatidate phosphatase are activated after TORC1 inactivation, supporting macroautophagy induction in the budding yeast Saccharomyces cerevisiae. On the other hand, whether and how microautophagy, which also consumes lipids, is regulated by TORC1 is controversial. Here we show that TORC1 inactivation induces microautophagy in budding yeast. Vps27, but not Atg1, Atg7, or Atg8, was required for TORC1 inactivation-induced microautophagy. Furthermore, the Nem1/Spo7-Pah1 axis was also critical for microautophagy induction. Thus, the TORC1-Nem1/Spo7-Pah1 axis is a master regulator of not only macroautophagy but also microautophagy in budding yeast.
营养饥饿和雷帕霉素靶蛋白复合物 1（TORC1）蛋白激酶失活促进巨自噬。巨自噬是一个消耗脂质的过程，在 TORC1 失活后，Nem1/Spo7 蛋白磷酸酶和 Pah1/lipin 磷酸二酯酶被激活，支持出芽酵母酿酒酵母中巨自噬的诱导。另一方面，TORC1 是否以及如何调节也消耗脂质的微自噬存在争议。在这里，我们表明 TORC1 失活会诱导出芽酵母中的微自噬。Vps27，但不是 Atg1、Atg7 或 Atg8，是 TORC1 失活诱导的微自噬所必需的。此外，Nem1/Spo7-Pah1 轴对于微自噬的诱导也很关键。因此，TORC1-Nem1/Spo7-Pah1 轴不仅是出芽酵母中巨自噬的主要调节剂，也是微自噬的主要调节剂。
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