Graduate School of Science and Technology, Shizuoka University, Japan.
Department of Science, Graduate School of Integrated Science and Technology, Shizuoka University, Japan.
FEBS J. 2018 May;285(10):1840-1860. doi: 10.1111/febs.14448. Epub 2018 Apr 16.
Autophagy is a process that requires intense membrane remodeling and consumption. The nutrient-responsive TORC1 (target of rapamycin complex 1) kinase regulates autophagy. However, how TORC1 controls autophagy via lipid/membrane biogenesis is unknown. TORC1 regulates the function of yeast phosphatidate phosphatase lipin Pah1 via the Nem1/Spo7 phosphatase complex. Here, we show that the Nem1/Spo7-Pah1 axis is required for autophagy induction after TORC1 inactivation and survival during starvation. Furthermore, this axis was critical for nucleophagy (both micronucleophagy and macronucleophagy) and was required for proper localization of micronucleophagy factor Nvj1 and macronucleophagy receptor Atg39. This study indicated that the Nem1/Spo7-Pah1 axis controlled by TORC1 is a critical branch for autophagy induction in nutrient starvation conditions.
自噬是一个需要剧烈的膜重塑和消耗的过程。营养感应的 TORC1（雷帕霉素复合物 1 的靶点）激酶调节自噬。然而，TORC1 如何通过脂质/膜生物发生来控制自噬尚不清楚。TORC1 通过 Nem1/Spo7 磷酸酶复合物调节酵母磷酸二酯酶磷酸酶 lipin Pah1 的功能。在这里，我们表明，在 TORC1 失活后诱导自噬和在饥饿期间存活时，Nem1/Spo7-Pah1 轴是必需的。此外，该轴对于核自噬（微核自噬和大核自噬）至关重要，并且对于微核自噬因子 Nvj1 和大核自噬受体 Atg39 的正确定位是必需的。这项研究表明，TORC1 控制的 Nem1/Spo7-Pah1 轴是营养饥饿条件下诱导自噬的关键分支。
