College of Veterinary Medicine/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China.
College of Veterinary Medicine/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China
Biosci Rep. 2018 Nov 13;38(6). doi: 10.1042/BSR20180742. Print 2018 Dec 21.
Myostatin gene () can inhibit the proliferation of myoblast, which in turn promotes muscle growth and inhibits adipocyte differentiation in livestock. MSTN mutation may lead to muscle hypertrophy or double-muscled (DM) phenotype. MSTN mutation animal, such as sheep, dog, and rabbit have been generated through CRISPR/Cas9 technology. However, goats with promising MSTN mutation have not been generated. We designed two sgRNAs loci targetting exon3 of gene to destroy the MSTN cysteines knots. We got seven goats from seven recipients, in which six were MSTN knocked-out (KO) goats, with a mutation rate of 85.7%. Destroyed cysteine knots caused MSTN structure inactivation. The average body weight gain (BWG) per day of MSTN KO goats was significantly higher than that of wild-type (WT) goats. MSTN KO goats showed abnormal sugar, fat, and protein metabolism compared with wild-type controls (MSTN). Inheritance of mutations was observed in offspring of MSTN KO goats by PCR analysis.
肌肉生长抑制素基因 () 可以抑制成肌细胞的增殖，从而促进家畜的肌肉生长和抑制脂肪细胞分化。MSTN 突变可能导致肌肉肥大或双肌（DM）表型。已经通过 CRISPR/Cas9 技术生成了具有 MSTN 突变的绵羊、狗和兔子等 MSTN 突变动物。然而，具有有前景的 MSTN 突变的山羊尚未产生。我们设计了两个靶向基因外显子 3 的 sgRNAs 位点，以破坏 MSTN 的半胱氨酸结。我们从 7 个受体中获得了 7 只山羊，其中 6 只是 MSTN 敲除（KO）山羊，突变率为 85.7%。破坏的半胱氨酸结导致 MSTN 结构失活。MSTN KO 山羊的日平均体重增加（BWG）明显高于野生型（WT）山羊。与野生型对照（MSTN）相比，MSTN KO 山羊表现出异常的糖、脂肪和蛋白质代谢。通过 PCR 分析观察到 MSTN KO 山羊后代的突变遗传。
