Hai Chao, Bai Chunling, Yang Lei, Wei Zhuying, Wang Hong, Ma Haoran, Ma Haibing, Zhao Yuefang, Su Guanghua, Li Guangpeng
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010070, China.
Sheng-Quan Ecological Animal Husbandry Company, Chifeng 024500, China.
Animals (Basel). 2023 Oct 14;13(20):3216. doi: 10.3390/ani13203216.
(1) Background: Myostatin (MSTN) is a protein that regulates skeletal muscle development and plays a crucial role in maintaining animal body composition and muscle structure. The loss-of-function mutation of gene can induce the muscle hypertrophic phenotype. (2) Methods: Growth indexes and blood parameters of the cattle of different months were analyzed via multiple linear regression. (3) Results: Compared with the control group, the body shape parameters of F2 cattle were improved, especially the body weight, cross height, and hip height, representing significant development of hindquarters, and the coat color of the F2 generation returned to the yellow of Luxi cattle. As adults, gene-edited bulls have a tall, wide acromion and a deep, wide chest. Both the forequarters and hindquarters are double-muscled with clear muscle masses. The multiple linear regression demonstrates that gene-edited hybrid beef cattle gained weight due to the higher height of the hindquarters. Significant differences in blood glucose, calcium, and low-density lipoprotein. Serum insulin levels decreased significantly at 24 months of age. gene editing improves the adaptability of cattle. (4) Conclusions: Our findings suggest that breeding with gene-edited Luxi bulls can improve the growth and performance of hybrid cattle, with potential benefits for both farmers and consumers.
(1) 背景：肌肉生长抑制素（MSTN）是一种调节骨骼肌发育的蛋白质，在维持动物身体组成和肌肉结构方面起着关键作用。该基因的功能丧失突变可诱导肌肉肥大表型。(2) 方法：通过多元线性回归分析不同月龄牛的生长指标和血液参数。(3) 结果：与对照组相比，F2代牛的体型参数得到改善，尤其是体重、体高和臀高，表明后躯显著发育，且F2代毛色恢复为鲁西黄牛的黄色。成年后，基因编辑公牛肩峰高且宽，胸部深且宽。前后躯均为双肌，肌肉块明显。多元线性回归表明，基因编辑杂交肉牛体重增加是由于后躯更高。血糖、钙和低密度脂蛋白存在显著差异。24月龄时血清胰岛素水平显著降低。基因编辑提高了牛的适应性。(4) 结论：我们的研究结果表明，用基因编辑的鲁西公牛进行育种可以提高杂交牛的生长性能，对养殖户和消费者都有潜在益处。
