Giovanelli Federica, Mattellini Matteo, Fichi Gianluca, Flamini Guido, Perrucci Stefania
Dipartimento di Scienze Veterinarie, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
Dipartimento di Farmacia, University of Pisa, Via Bonanno Pisano 6, 56126 Pisa, Italy.
Vet Sci. 2018 Sep 10;5(3):78. doi: 10.3390/vetsci5030078.
By using the egg hatch test (EHT), the larval development test (LDT) and the larval mortality/paralysis test (LMT), the anthelmintic activity on sheep gastrointestinal strongyles (GIS) of four plant-derived pure compounds, mangiferin (at 0.25%, 0.125% and 0.0625%), rutin (at 1%, 0.75%, 0.5%), quercetin (at 1%), and β-sitosterol (at 1%, 0.75%, 0.5%), was investigated. For comparison, untreated and treated (0.1% thiabendazole, 0.1% TBZ) controls were used. Six repetitions were made throughout the experiment. Data were statistically elaborated using the χ² test. The concentration able to inhibit the development of the 50% of L1s to L3s and causing the mortality of the 50% of L3s (EC50) was also calculated. L3s recovered from untreated Petri dishes were identified at the genus level. In EHT, all tested compounds at all concentrations significantly ( < 0.01) inhibited the hatch of the eggs when compared to the untreated controls, but none of them was as effective as 0.1% TBZ. In LDT, rutin (at 1%, 0.75% and 0.5%), mangiferin (at 0.25% and 0.125%), β-sitosterol (at 1%) and 0.1% TBZ completely prevented the larval development from L1 to L3 in respect to the untreated controls ( < 0.01). In LMT, all tested compounds significantly ( < 0.01) increased the death of L3s compared to the untreated controls, except for β-sitosterol at 0.5%. However, only rutin at all concentrations and 0.25% and 0.125% mangiferin were as effective as 0.1% TBZ. , , and GIS genera, were identified.
通过使用虫卵孵化试验（EHT）、幼虫发育试验（LDT）和幼虫死亡率/麻痹试验（LMT），研究了四种植物源纯化合物（芒果苷，浓度分别为0.25%、0.125%和0.0625%；芦丁，浓度分别为1%、0.75%、0.5%；槲皮素，浓度为1%；β-谷甾醇，浓度分别为1%、0.75%、0.5%）对绵羊胃肠道圆线虫（GIS）的驱虫活性。作为对照，设置了未处理对照组和处理组（0.1%噻苯达唑，0.1% TBZ）。整个实验进行了六次重复。数据采用χ²检验进行统计学处理。还计算了能够抑制50%的L1发育为L3并导致50%的L3死亡的浓度（EC50）。从未处理培养皿中回收的L3在属水平上进行鉴定。在虫卵孵化试验中，与未处理对照组相比，所有浓度的受试化合物均显著（<0.01）抑制了虫卵孵化，但它们均不如0.1% TBZ有效。在幼虫发育试验中，芦丁（浓度为1%、0.75%和0.5%）、芒果苷（浓度为0.25%和0.125%）、β-谷甾醇（浓度为1%）和0.1% TBZ相对于未处理对照组（<0.01）完全阻止了幼虫从L1发育为L3。在幼虫死亡率/麻痹试验中，与未处理对照组相比，除0.5%的β-谷甾醇外，所有受试化合物均显著（<0.01）增加了L3的死亡。然而，只有所有浓度的芦丁以及0.25%和0.125%的芒果苷与0.1% TBZ效果相当。鉴定出了 、 、 和GIS属。
