Laviolette M, Picard S, Braquet P, Borgeat P
Prostaglandins Leukot Med. 1986 Aug;23(2-3):191-9. doi: 10.1016/0262-1746(86)90185-x.
The arachidonic acid lipoxygenase metabolites of polymorphonuclear leukocyte (PMNL) preparations obtained from 31 subjects with eosinophilia (eosinophil content of PMNL preparations: 27.0%, 4-73, median with range) and 29 normal donors (4.5%, 0-15) were analyzed by reversed phase high performance liquid chromatography. More leukotriene (LT) C4 and 15-hydroxyeicosatetraenoic acid (HETE) (p less than 0.001) and less LTB4 (p less than 0.005) were found in eosinophil-rich preparations in comparison to controls on incubations with ionophore and arachidonic acid. In incubations with arachidonic acid alone, only small amounts of 5-lipoxygenase metabolites were produced by both groups, but the PMNL preparations from patients with eosinophilia showed higher capacity to release LTC4 and 15-HETE (p less than 0.005). In the eosinophil-rich preparations, the percentage of eosinophils correlated positively with the production of LTC4 and negatively with LTB4 (p less than 0.05, incubations with ionophore +/- arachidonic acid), and positively with 15-HETE (p less than 0.01, incubations with arachidonic acid +/- ionophore). Moreover the eosinophil-rich preparations produced more LTC4 per eosinophil in incubations with arachidonic acid alone (p less than 0.001) than normal PMNL preparations. The predominant release of LTC4 and 15-HETE by eosinophils was further confirmed by the analysis of bronchoalveolar lavage cells. Lavage cells containing eosinophils released LTC4 and 15-HETE while normal bronchoalveolar lavages, which were poor in eosinophils, did not produce detectable amount of LTC4 and 15-HETE. These results show that eosinophils release preferentially LTC4 and 15-HETE in contrast to LTB4 for the neutrophils.
采用反相高效液相色谱法分析了从31名嗜酸性粒细胞增多症患者（中性粒细胞制备物中的嗜酸性粒细胞含量：27.0%，4 - 73，中位数及范围）和29名正常供体（4.5%，0 - 15）获取的中性粒细胞（PMNL）制备物的花生四烯酸脂氧合酶代谢产物。与对照组相比，在与离子载体和花生四烯酸孵育时，富含嗜酸性粒细胞的制备物中发现更多的白三烯（LT）C4和15 - 羟基二十碳四烯酸（HETE）（p < 0.001），而白三烯B4（LTB4）较少（p < 0.005）。仅与花生四烯酸孵育时，两组均仅产生少量的5 - 脂氧合酶代谢产物，但嗜酸性粒细胞增多症患者的PMNL制备物显示出更高的释放LTC4和15 - HETE的能力（p < 0.005）。在富含嗜酸性粒细胞的制备物中，嗜酸性粒细胞的百分比与LTC4的产生呈正相关，与LTB4呈负相关（p < 0.05，与离子载体±花生四烯酸孵育），与15 - HETE呈正相关（p < 0.01，与花生四烯酸±离子载体孵育）。此外，仅与花生四烯酸孵育时，富含嗜酸性粒细胞的制备物中每个嗜酸性粒细胞产生的LTC4比正常PMNL制备物更多（p < 0.001）。支气管肺泡灌洗细胞的分析进一步证实了嗜酸性粒细胞优先释放LTC4和15 - HETE。含有嗜酸性粒细胞的灌洗细胞释放LTC4和15 - HETE，而嗜酸性粒细胞较少的正常支气管肺泡灌洗则未产生可检测量的LTC4和15 - HETE。这些结果表明，与中性粒细胞释放LTB4相反，嗜酸性粒细胞优先释放LTC4和15 - HETE。
