Borgeat P, Fruteau de Laclos B, Rabinovitch H, Picard S, Braquet P, Hébert J, Laviolette M
J Allergy Clin Immunol. 1984 Sep;74(3 Pt 2):310-5. doi: 10.1016/0091-6749(84)90122-2.
Blood samples were obtained from a group of 20 patients with hypereosinophilia (greater than or equal to 1500 eosinophils/mm3). The polymorphonuclear leukocytes (PMNLs) were prepared from blood treated with ethylenediaminetetra-acetic acid by successive dextran sedimentation of the red blood cells, separation of mononuclear leukocytes and PMNLs on Ficoll-Paque, and ammonium chloride treatment of the PMNL fraction. The eosinophil content of the final PMNL preparations ranged from 15% to 75%, as assessed by Wright-stained smears, and the remaining leukocytes were predominantly neutrophils with only 3% to 5% mononuclear cells. The eosinophil-rich PMNL preparations as well as PMNL preparations from normal volunteers were incubated under various conditions and the arachidonic acid metabolites were analyzed by reverse-phase high-performance liquid chromatography. The synthesis of 5-lipoxygenase products was strongly stimulated by the ionophore A23187 in both normal and eosinophil-rich PMNL preparations. Whereas the normal PMNL preparations, which were eosinophil poor, produced 10 to 25 times more leukotriene B4 than leukotriene C4, the eosinophil-rich PMNL preparations characteristically released leukotriene C4 in equal or up to 20 times greater amounts than leukotriene B4.
从一组20例嗜酸性粒细胞增多症患者（嗜酸性粒细胞≥1500/mm³）采集血样。通过对用乙二胺四乙酸处理的血液进行连续的红细胞葡聚糖沉降、在Ficoll-Paque上分离单核白细胞和多形核白细胞（PMNL）以及对PMNL部分进行氯化铵处理来制备多形核白细胞。通过瑞氏染色涂片评估，最终PMNL制剂中的嗜酸性粒细胞含量为15%至75%，其余白细胞主要是中性粒细胞，单核细胞仅占3%至5%。将富含嗜酸性粒细胞的PMNL制剂以及来自正常志愿者的PMNL制剂在各种条件下孵育，并通过反相高效液相色谱分析花生四烯酸代谢产物。在正常和富含嗜酸性粒细胞的PMNL制剂中，离子载体A23187均强烈刺激5-脂氧合酶产物的合成。嗜酸性粒细胞较少的正常PMNL制剂产生的白三烯B4比白三烯C4多10至25倍，而富含嗜酸性粒细胞的PMNL制剂的特征是释放的白三烯C4与白三烯B4相等或比白三烯B4多20倍。
