Postgraduate Program of Cellular and Molecular Biology Applied to Health Sciences (PPGBioSaúde), Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
Basic Health Science, University of the Campanha Region (URCAMP), Bagé, RS, Brazil.
Oxid Med Cell Longev. 2018 Aug 19;2018:7804135. doi: 10.1155/2018/7804135. eCollection 2018.
The use in folk medicine of and recent studies on DNA damage by oxidative stress mechanisms have motivated this study. We investigated the biotoxicological effects of trimeroside from this plant. Aqueous extract from aerial parts of was fractioned by flash chromatography for further isolation by thin-layer chromatography. The novel nor-monoterpene glycoside, trimeroside, and three flavonoids, cirsimaritin, luteolin and quercetin, were isolated. The genotoxic and mutagenic potential of trimeroside was determined by /microsome (TA98 and TA100), comet assay, and cytokinesis-block micronucleus cytome assay (CBMN-cyt) in HepG2 cells. We also screened trimeroside into different human tumoral cell lines by sulforhodamine B (SRB) assay. Mutagenicity was detected in TA100 strain with metabolic activation. Genotoxic effects were not observed in HepG2 by comet assay. However, a decrease in the nuclear index division in the 2.0 mg·mL concentration and an increase of nucleoplasmic bridges in the 1.5 mg·mL concentration were detected by CBMN-cyt assay indicating cytotoxic and mutagenic effects. In SRB assay, trimeroside showed weak antiproliferative activity against the cell lines.
民间医学的应用和最近关于氧化应激机制引起的 DNA 损伤的研究促使我们进行了这项研究。我们研究了这种植物的三萜苷的生物毒性作用。通过闪式色谱法对植物地上部分的水提物进行了分离，然后通过薄层层析进一步分离。分离得到了新型的单萜糖苷三萜苷和三种类黄酮，包括朝霍定、木樨草素和槲皮素。通过 /微粒体（TA98 和 TA100）、彗星试验和胞质分裂阻断微核细胞试验（CBMN-cyt）在 HepG2 细胞中测定了三萜苷的遗传毒性和致突变性。我们还通过磺酰罗丹明 B（SRB）试验筛选了三萜苷进入不同的人肿瘤细胞系。代谢活化时在 TA100 菌株中检测到致突变性。彗星试验未在 HepG2 中观察到遗传毒性。然而，CBMN-cyt 试验检测到在 2.0mg·mL 浓度下核指数分裂减少，在 1.5mg·mL 浓度下核质桥增加，表明具有细胞毒性和致突变性。在 SRB 试验中，三萜苷对细胞系表现出较弱的增殖抑制活性。
